母体暴露壬基酚对仔鼠脾脏组织的氧化损伤  

Toxicity of Nonylphenol on F1 Rats' Spleen after Gestation Exposure

作　　者：许洁[1,2] 俞捷[2] 汪洋[1] 张子萍[2] 张洋[2] 吴伟成[2] 

机构地区：[1]重庆医科大学公共卫生学院,重庆400010 [2]遵义医学院预防医学教研室,贵州遵义563003

出　　处：《环境与职业医学》2010年第6期366-367,370,共3页Journal of Environmental and Occupational Medicine

基　　金：贵州省自然科学基金(编号:黔科2019号);遵义市自然科学基金(编号:遵市科合社字200805);遵义医学院院基金(编号:2007-61-22)

摘　　要：[目的]研究母体暴露壬基酚(NP)对仔鼠脾脏的氧化损伤。[方法]将SD母鼠随机分为50、100、200mg/kgNP暴露组及对照组,每组6～7只动物。在母鼠妊娠第9～15天灌胃NP,仔鼠(F1)于出生60天龄时处死并取脾脏称重。比色法测定脾脏组织中超氧化物歧化酶(SOD)活力及脂质过氧化产物丙二醛(MDA)含量;透射电镜观察其超微结构改变。[结果]与对照组比较,200mg/kgNP组仔鼠的脾脏重量及脏器系数均明显降低(P<0.05),且与染毒剂量呈负相关(r脾=-0.618,r脏=-0.530,P<0.05);100、200mg/kgNP组仔鼠脾脏组织的SOD活力降低,200mg/kgNP组MDA含量升高(P<0.05),均存在剂量效应关系(rSOD=-0.460,rMDA=0.747,P<0.05)。高暴露组仔鼠脾脏电镜下可见脾细胞线粒体肿胀呈空泡样变,染色质浓缩成块状聚集在核周围。[结论]NP可透过胎盘屏障致仔鼠脾脏脂质过氧化损伤。[ Objective ] To investigate the adverse effects on F] rats＇spleen while the pregnant mother rats were exposed to nonylphenol （ NP ）. [ Methods ] Pregnant rats were exposed to NP via gavage at doses of 0, 50, 100 and 200 mg/kg by body weights respectively per day, from pregnant days 9 to 15. The level of malondialdehyde（ MDA ）and activity of super oxide dimutese （ SOD ） in FI rats＇ spleen homogenate were detected respectively at the age of 60th day. The ultrastructure of spleen tissues were observed under electronic microscope. [ Results ] Compared with the control group, the absolute and relative weight of spleen were decreased dramatically in the 200 mg/kg NP treated group and negative relation between the weight and dose was found （ rSOD=-0.618, rcoefficient=-0.530, P〈0.05 ）. The SOD activity of spleen tissues decreased in NP I00 and 200 mg/kg exposed fetal rats, while the levels of MDA increased in NP 200mg/kg exposed fetal rats. Both of them were in a dose-dependent manner （rSOD=-0.460, rMDA=0.747, P〈0.05 ）. The differences mentioned above were statistical significant compared to control group（ P〈0.05 ）. The changes of the uhrastructrue were found among the spleen of NP 200 mg/kg group that characterized with ehromatin condesed, clumped in circa-nuclei and mitochondrial tumefaction and vaeuolization. [ Conclusion ] NP can get across the placenta barrier to make the F1 offspring rats＇spleen damage of lipid peroxidation.

关 键 词：壬基酚 仔鼠 脾脏 脂质过氧化 

分 类 号：R114[医药卫生—卫生毒理学]