液相色谱-串联质谱法同时检测饲料中7种精神类药物  

Simultaneous Determination of Seven Mental Drugs in Feeds by Liquid Chromatography-Tandem Mass Spectrometry

作　　者：索德成[1] 赵根龙 李兰[1] 苏晓鸥[1] 

机构地区：[1]中国农业科学院农业质量标准与检测技术研究所,北京10081

出　　处：《分析化学》2010年第7期1023-1026,共4页Chinese Journal of Analytical Chemistry

基　　金：国家"十一五"科技支撑计划项目基金(No.2006BAD12B03)支持

摘　　要：建立了液相色谱-串联质谱同时检测饲料样品中7种精神类药物(硝西泮、奥沙西泮、氯丙嗪、异丙嗪、地西泮、奋乃静、硫利达嗪)的方法。通过对提取溶剂、净化等预处理条件及LC-MS/MS分析条件的优化,可以同时检测饲料中7种违禁精神类药物。饲料样品经乙腈/水(9:1,V/V)提取后,过MCX固相萃取柱净化,氮吹至干,用1mL乙腈/水(2:8,V/V)溶解后测定,采用SRM模式进行定性与定量分析。7种精神类药物在饲料中的回收率为53.9%～110.2%;相对标准偏差为3.4%～18.4%;硝西泮、奥沙西泮、氯丙嗪、异丙嗪的检出限为1.0ng/g;对地西泮、奋乃静、硫利达嗪的检出限为5.0ng/g。结果表明,本方法可用于饲料中7种精神类药物的测定。A method was established for the simultaneous determination of seven mental drugs by LC-MS/MS.In this method,the conditions of appropriate extracting solvent,SPE cartridge and LC-MS/MS were optimized.The drugs were extracted with acetonitrile/water（9：1,V/V）,and then purified by MCX solid-phase extraction （SPE） column.The elution was evaporated by nitrogen blow,dissolved in acetonitrile/water（2：8,V/V） and analyzed by LC-MS/MS.The limit of detection （LOD） was 1.0 ng/g of nitrazepam,oxazepam,promethazine and chlorpromazine,and 5.0 ng/g of diazepam,perphenazine;and thioridazine.The recoveries were 53.9%-110.2%,and RSDs were 3.4%-18.4%.Results showed that this developed method was very reliable for the determination of seven mental drugs in feeds.

关 键 词：液相色谱-串联质谱 饲料 精神类药物 

分 类 号：S816.17[农业科学—饲料科学]