基于量子点的荧光共振能量转移  

Fluorescence Resonance Energy Transfer Between Quantumn Dots

作　　者：刘海燕[1] 吴盛美[1] 程芳[1] 胡育筑[1,2] 严拯宇[1,2] 

机构地区：[1]中国药科大学分析化学教研室 [2]中国药科大学药物质量与安全预警教育部重点实验室,南京210009

出　　处：《分析化学》2010年第7期1036-1039,共4页Chinese Journal of Analytical Chemistry

基　　金：国家自然科学基金(No.30873197)资助项目

摘　　要：研究了具有相反电荷的两种量子点间的荧光共振能量转移。分别以巯基乙酸(TGA)和十六烷基三甲基溴化胺(CTMAB)修饰发射绿色和红色荧光的CdSe/ZnS量子点,使其由油溶性变为水溶性,且表面带相反的电荷,并对修饰后的水相量子点进行琼脂糖凝胶电泳、荧光成像、量子产率等系列表征。对两种量子点间的荧光共振能量转移现象进行研究。结果表明:在激发波长为400nm时,两种量子点在磷酸盐缓冲溶液(pH7.5)中具有较好的荧光共振能量转移效率(猝灭效率0.54,增强效率0.27)。The fluorescent resonance energy transfer（FRET） between two kinds of quantum dots（QDs） with different surface-charge was researched.Green-emission QDs was modified with amphiphilic surfactants（CTMAB） and red-emission QDs was modified with thiolglycolic acid（TGA） separately.The experiments proved that both CTMAB and TGA both could well turn the oil-soluble QDs into water-soluble ones,as well as opposite electric charges on the surface.Then the water-soluble QDs were characterized by agarose gel electrophoresis,fluorescence image and quantum yield.Taking advantage of the interaction between the opposite charges,fluorescent resonance energy transfer（FRET） was detected between two kinds of QDs in phosphate buffer solution（pH 7.5） with an excitation wavelength of 400 nm,and the FRET efficiencies were satisfied（quenching efficiency was 0.54 and enhance efficiency was 0.27）.

关 键 词：量子点 十六烷基三甲基溴化胺 巯基乙酸 荧光共振能量转移 

分 类 号：O641.121[理学—物理化学]