腺苷动力学与肝脏缺血预处理关系的研究  

Effects of Adenosine in Ischemic Preconditioning of Rat Livers

作　　者：欧阳兵[1] 许元鸿[2] 王晨超[2] 

机构地区：[1]辽宁省沈阳市第六人民医院普外科,110006 [2]辽宁省沈阳市中国医科大学附属第一医院,110001

出　　处：《医学信息（下旬刊）》2010年第7期1-3,共3页Medical information

基　　金：辽宁省自然基金资助项目（20042077）

摘　　要：目的：探讨腺苷动力学与缺血预处理（Ischemia preconditioning IPC）所产生的肝脏保护功能的关系。方法：建立大鼠肝脏热缺血再灌注模型。IPC采用肝脏缺血10min，再灌注10min。经过45min缺血后再灌注，用酶学方法测量肝组织中腺苷浓度变化、血清酶学指标（ALT、AST、LDH）变化和肝脏病理组织学变化。结果：IPC组腺苷浓度较对照组明显升高，血清酶学指标明显降低，肝组织充血明显减轻。结论：腺苷的动力学变化与IPC引起肝脏的保护功能有关。Objective To investigate the relationship between the dynamics of adenosine and ischemic tolerance resulted from ischemic precondition- ing（IPC） in rat. Methods Male SD rats were used in our experiment. IPC group was administrated with 10--minute ischemia and then 10--minute reperfusion. During long--period ischemia and reperfusion, liver tissue and blood samples were collected at specific time. Concentration of adenosine, serum levels of liver--related enzymes were evaluated. Results Adenosine concentration increased dramatically in IPC group（P〈0.05） during 45--minute ischema and subsequent reperfusion. Serum levels of liver--related enzymes descended in IPC group（P〈0.05） during reperfusion. Conclusion dy- namics of adenosine is related to ischemic tolerance resulted from IPC. Increase of adenosine concentration was detected immediately after IPC and re- mained at a high level. This demonstrated the linkage between adenosine and early preconditioning.

关 键 词：再灌流损伤 缺血预处理 腺苷 大鼠 

分 类 号：R617.1[医药卫生—外科学]