腺苷动力学与肝脏缺血预处理关系的研究  

Effects of Adenosine in Ischemic Preconditioning of Rat Livers

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作  者:欧阳兵[1] 许元鸿[2] 王晨超[2] 

机构地区:[1]辽宁省沈阳市第六人民医院普外科,110006 [2]辽宁省沈阳市中国医科大学附属第一医院,110001

出  处:《医学信息(下旬刊)》2010年第7期1-3,共3页Medical information

基  金:辽宁省自然基金资助项目(20042077)

摘  要:目的:探讨腺苷动力学与缺血预处理(Ischemia preconditioning IPC)所产生的肝脏保护功能的关系。方法:建立大鼠肝脏热缺血再灌注模型。IPC采用肝脏缺血10min,再灌注10min。经过45min缺血后再灌注,用酶学方法测量肝组织中腺苷浓度变化、血清酶学指标(ALT、AST、LDH)变化和肝脏病理组织学变化。结果:IPC组腺苷浓度较对照组明显升高,血清酶学指标明显降低,肝组织充血明显减轻。结论:腺苷的动力学变化与IPC引起肝脏的保护功能有关。Objective To investigate the relationship between the dynamics of adenosine and ischemic tolerance resulted from ischemic precondition- ing(IPC) in rat. Methods Male SD rats were used in our experiment. IPC group was administrated with 10--minute ischemia and then 10--minute reperfusion. During long--period ischemia and reperfusion, liver tissue and blood samples were collected at specific time. Concentration of adenosine, serum levels of liver--related enzymes were evaluated. Results Adenosine concentration increased dramatically in IPC group(P〈0.05) during 45--minute ischema and subsequent reperfusion. Serum levels of liver--related enzymes descended in IPC group(P〈0.05) during reperfusion. Conclusion dy- namics of adenosine is related to ischemic tolerance resulted from IPC. Increase of adenosine concentration was detected immediately after IPC and re- mained at a high level. This demonstrated the linkage between adenosine and early preconditioning.

关 键 词:再灌流损伤 缺血预处理 腺苷 大鼠 

分 类 号:R617.1[医药卫生—外科学]

 

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