无花果曲霉(Aspergillus ficuum 3.4322)植酸酶基因的克隆及序列分析  

Cloning and Analysis of Phytase gene from Aspergillus ficuum 3.4322

作　　者：张波[1] 王林美[1] 叶博[1] 赵振军[1] 李树英[1] 

机构地区：[1]辽宁省农业科学院大连生物技术研究所,辽宁大连116024

出　　处：《辽宁农业科学》2010年第3期22-25,共4页Liaoning Agricultural Sciences

摘　　要：以无花果曲霉(Aspergillus ficuum 3.4322)基因组DNA为模板,用PCR方法扩增植酸酶基因(phyA)。将PCR产物克隆到pMD 18-T质粒中,用于DNA测序。DNA序列分析结果表明,基因全长1 509 bp,其中包括一个长105 bp的内含子。编码467个氨基酸,第1-19氨基酸为信号肽序列,成熟的植酸酶由448个氨基酸组成。与黑曲霉(Aspergillus niger)植酸酶(GenBank Accession:M94550)的氨基酸同源性为95.7%,与无花果曲霉(Aspergillus ficuum3.324)植酸酶(GenBank Accession:AY013315)的氨基酸同源性为98.9%,显示了植酸酶基因在不同菌株中的差异。A Phytase gene（phyA） from Aspergillus ficuum 3.4322 was cloned by PCR using genomic DNA as template.The PCR products were cloned into pMD18-T vector for sequencing.The sequencing results showed that the full length of phyA is 1509bp with an intron of 105bp.It encodes a protein of 467 amino acids.There is a signal peptide of 19 amino acids at the N-terminus.The mature protein of Phytase from Aspergillus ficuum 3.4322 consists of 448 amino acids.The amino acid homology of Phytase from Aspergillus ficuum 3.4322 is 95.7% and 98.9% compared with Phytase from Aspergillus niger（GenBank Accession： M94550） and Aspergillus ficuum 3.324（GenBank Accession： AY013315）,respectively.It shows the diversity of Phytase gene in different strains.

关 键 词：无花果曲霉 植酸酶 基因克隆 

分 类 号：Q785[生物学—分子生物学]