猪肺炎支原体不同毒力株黏附因子基因序列分析  

Sequence Analysis of Adhesin Gene of Different Mycoplasma hyopneumoniae Strains

作　　者：刘茂军[1] 邵国青[1] 张映[2] 

机构地区：[1]江苏省农业科学院兽医研究所,江苏南京210014 [2]山西农业大学动物科技学院,山西太谷030801

出　　处：《江苏农业学报》2010年第3期522-525,共4页Jiangsu Journal of Agricultural Sciences

基　　金：农业科技成果转化基金项目(2009GB2C100129);公益性行业(农业)科研专项(200803020);江苏省农业科技自主创新基金项目[CX(09)118]

摘　　要：将猪肺炎支原体不同毒力株培养获得菌体,提取基因组DNA,PCR扩增黏附因子P97基因,对PCR产物进行测序,将猪肺炎支原体不同毒力株的P97基因序列的碱基组成和推导的氨基酸序列进行比较,分析猪肺炎支原体黏附因子P97基因变化与致病性的关系。结果表明,猪肺炎支原体黏附因子P97基因与菌株致病性有一定关系,但除P97基因之外,还有其他与致病有关的基因存在。The genomic DNA was extracted from different attenuated Mycoplasma hyopneumoniae strains.The adhesion gene P97 were amplified by PCR,and then were sequenced.The base composition of DNA sequences of P97 and amino acid sequence deduced from different attenuated strains of Mycoplasma hyopneumoniae were compared,and the relationship between genetic variation and virulence was analyzed.The results showed that there was some relationship between genetic variation and virulence of Mycoplasma hyopneumoniae.But there were other genes associated with virulence besides P97.

关 键 词：猪肺炎支原体 黏附因子 毒力 序列 

分 类 号：Q78[生物学—分子生物学]