微囊藻毒素降解菌EMB分离鉴定及其降解特性  

Identification and Characterization of Microcystin-degrading Strain EMB

作　　者：尹玉芬[1,2] 胡梁斌[3] 周威[2] 杨静东[2,4] 周益军[1,2] 石志琦[2] 

机构地区：[1]南京农业大学植物保护学院,农业部作物病虫害监测与防控重点开放实验室,江苏南京210095 [2]江苏省农业科学院食品质量安全与检测研究所,江苏南京210014 [3]河南科技学院食品学院,河南新乡453003 [4]江苏省东海县农业局,江苏东海222300

出　　处：《江苏农业学报》2010年第3期631-636,共6页Jiangsu Journal of Agricultural Sciences

基　　金：国家“973”预研项目(2008CB117001)

摘　　要：以藻毒素提取液作为唯一碳源和氮源,从太湖蓝藻堆积点底泥中分离筛选高效微囊藻毒素降解菌。结果表明:经过驯化筛选得到1株高效降解菌EMB。在培养温度30℃、pH值为7时其降解毒素效率最高,培养21h内可将初始浓度为2.99 mg/L和2.15 mg/L的MC-RR和MC-LR完全降解,此外菌株EMB可以将太湖水华样品中的MC-RR和MC-LR在2周内降解到安全饮用水标准范围内。形态、生理特性和16 S rDNA序列分析表明,菌株EMB属于芽孢杆菌属(Bacillus.sp.),与已报道的B.subtilisB-FS06在进化树上距离最近。A microcystins-degrading strain was isolated from activated mud of blue-green algae stacking pot of Taihu Lake through acclimatization with microcystins（MCs） extract from algae powder as sole carbon and nitrogen sources.The strain EMB performed the most efficient MCs-biodegradation when incubated at 30 ℃ and pH 7.0.2.99 mg/L MC-RR and 2.15 mg/L MC-LR were completely degraded by strain EMB within 21 h.Additionally,the concentrations of MC-RR and MC-LR in the water bloom sample from the Taihu Lake decreased to the standard of safe drinking water by applying strain EMB for 2 weeks.The strain EMB was identified as Bacillus sp.which was the closest to the B.subtilis B-FS06 in the phylogenic tree by morphological and physiological properties and 16S rDNA sequence analysis.

关 键 词：微囊藻毒素 芽孢杆菌 生物降解 16S RDNA 

分 类 号：S182[农业科学—农业基础科学]