转化生长因子β_1表达在小鼠放射性肺纤维化形成过程中的作用  

The role of TGF β 1 expression in the formation of lung fibrosis in mice after thoracic irradiation

作　　者：崔晓利[1,2] 刘海玲[1,2] 董秀怠 殷蔚伯 

机构地区：[1]天津医科大学附属肿瘤医院 [2]中国医学科学院中国协和医科大学肿瘤医院

出　　处：《中华放射肿瘤学杂志》1999年第1期47-49,共3页Chinese Journal of Radiation Oncology

摘　　要：目的探讨转化生长因子β１（ＴＧＦβ１）在小鼠放射性肺纤维化形成过程中表达变化及作用。方法雄性Ｃ５７ＢＬ小鼠，全肺１次照射１３Ｇｙ，不同时间取肺组织ＨＥ染色、Ｍａｓｏｎ染色、ＶＧ染色观察肺纤维化形成。逆转录聚合酶链反应（ＲＴＰＣＲ）方法测定肺组织内ＴＧＦβ１的表达。结果照射后８个月局部灶性纤维化形成。照射后３６小时ＴＧＦβ１表达下降；照射后２个月ＴＧＦβ１表达升高；至照射后８个月，略呈下降趋势，但仍高于正常对照。结论ＴＧＦβ１的表达在肺纤维化形成过程中起重要作用。Objective To study the role of expression of TGF β 1 in the formation of lung fibrosis in mice after thoracic irradiation.Methods Using the lungs of C57BL mice,we observed the histological changes stained with HE,Masson and VG up to 8 months after whole thorax irradiation.Expression of TGF β 1 was evaluated by RT PCR technique.Results The expression of TGF β 1 decreased at H 36 after whole thoracic irradiation,increased in 2 months and remained elevated until 8 months when lung fibrosis appeared.Conclusions These studies clearly demonstrate that alterations in expression of TGF β 1 occur very early after pulmonary irradiation and may play a role in the development of chronic fibrosis

关 键 词：转化生长因子 肺纤维化 放射性 病理 

分 类 号：R818.841[医药卫生—放射医学]