豚鼠耳蜗一氧化氮合酶组织化学反应的定量观察  

Quantitative Observation on Histochemical Relativity of Nitric Oxide Synthase in Guinea Pig Cochlea

作　　者：沈静[1,2] 汤浩[1,2] 方秀斌 张合[1,2] 章晓芳[1,2] 张铁民 

机构地区：[1]中国医科大学听觉研究室 [2]中国医科大学脑研究所神经解剖室

出　　处：《解剖科学进展》1999年第1期40-43,共4页Progress of Anatomical Sciences

摘　　要：应用ＮＡＤＰＨ—ｄ酶组化方法结合显微图像分析技术，定量观察了正常豚鼠耳蜗ＮＯＳ的分布及活性。结果表明：ＮＯＳ存在于豚鼠外周听觉系统，分布部位依其活性强弱依次为血管纹、内毛细胞、螺旋神经节、外毛细胞、听神经纤维，其相应部位的ＮＯＳ阳性反应物的平均密度值为９３．９２％、９１．１７％、８４．２５％、７８．７５％、３２．９２％，平均灰度值为６６．１８、７３．４２、７６．６７、８５．１７、１１１．３３。The distribution of NO synthase (NOS) was quantitatively studied by means of NADPH-diaphorase histochemistry staining combined with image quantitative analysis technique in normal guinea pig cochlea. The results showed that the NO synthase was present in the peripheral auditory system. Based on extent of NO synthase activity, the region of distribution in turn were stria vascularis, inner hair cells, outer hair cells , spiral ganglion cells and auditory nerve fibers. The average densities of above regions were 93.92%, 91.71%, 84.25%, 78.75% and 32.92% respectively, while the average grey level of above regions were 66.18, 73.42, 76.67, 85.17 and 111.33 respectively. The results suggest that NO in the peripheral auditory system is related to the auditory modulation and might play an improtant role in the physiology and pathophysiology of the mammalian cochlea. The characteristics and the physiological significance of NO synthase distribution in the cochlea were discaussed.

关 键 词：一氧化氮合酶 耳蜗 组织化学 

分 类 号：R329－33[医药卫生—人体解剖和组织胚胎学] R329.26[医药卫生—基础医学]