新的免疫调节剂筛选模型的建立  

The Establishment of a Screening Method for Immunoregulators

作　　者：张祝兰[1] 林善[1] 唐文力[1] 王清波[1] 洪金基[1] 

机构地区：[1]国家医药管理局

出　　处：《海峡药学》1999年第1期13-15,共3页Strait Pharmaceutical Journal

基　　金：本项目受福建省自然科学基金

摘　　要：本文报道利用小鼠脾淋巴细胞体外培养法建立一种新的免疫调节剂的筛选模型。探讨并确立了脾淋巴细胞浓度、刀豆素蛋白浓度，甲■溶解剂及培养方式等影响因素，实验证明该模型对已知的免疫调节剂是敏感的，本方法具有简便、快速、敏感及对设备要求低等优点，适用于一般实验室免疫调节剂的筛选。This paper describes the establishment of a screening method for immunoregulators,which uses the spleen lymphocyte of mouse as target cell. We use MTT assay to determine the account of cells.We research the factors that coruprise the concentration of spleen lymphocyte the concentration of ConA,MTT-for mazan lysing buffer and cuIture pattern. The experiment proves that this method is Convenient,fast, sensitive and not need special equipment. It can be applicable to gereral laboratory to screen immunoregulators.

关 键 词：免疫调节剂 筛选模型 MTT检测 

分 类 号：R979.5[医药卫生—药品] R965.1[医药卫生—药学]