孕中期时间分辨荧光法检测血清标志物在产前筛查中的应用  

Application of DELFIA for detecting maternal serum biochemical markers in second trimestry on antenatal screening

作　　者：杨延冬[1] 王莉[1] 姜秀清[1] 蔺香云[1] 韩兆东[1] 

机构地区：[1]滨州医学院附属医院产科,山东滨州256603

出　　处：《现代医药卫生》2010年第14期2085-2087,共3页Journal of Modern Medicine & Health

基　　金：山东省人口计生委科技课题:编号200808

摘　　要：目的:探讨时间分辨荧光免疫检测技术(DELFIA)在孕中期的唐氏综合征、18-三体综合征筛查中的应用。方法:收集1976例妊娠妇女孕中期(15～20周)血清标本,应用DELFIA及配套的统计软件,检测hAFP、游离β-绒毛膜促性腺激素(F-βHCG),结合临床有关资料进行分析。结果:1 976例筛查孕妇中,母血清检测DS高危94例,18-三体综合征高危4例,21-三体综合征3例,18-三体综合征1例,另外6例染色体正常的其他胎儿异常。结论:采用DELFIA联合检测hAFP和Free-βHCG可以评估胎儿患唐氏综合征、18-三体综合征的风险,显著降低需进行创伤性产前诊断的孕妇比例,是目前较为可行、容易被孕妇接受的唐氏综合征产前筛查技术。Objective：To explore the application value of dissociation enhanced lanthanide fluoroimmunoassay（DELFIA） in prenatal screening of Down’s syndrome（DS） and 18-trisomy syndrome on middle period pregnancy.Methods：The serum AFP and F-βHCG of 1976 cases in the second trimester（15～20 weeks） pregnant women were quantitatively detected by DELFIA and corresponding statistical software.After the examination,the results were calculated and combining related data to analyze fetal risk of 21-trisomy syndrome and 18-trisomy syndrome.Results：1976 pregnant women accepted antenatal screening,94 cases of them were at high risk with 21-trisomy syndrome,4 cases were detected at high risk with 18-trisomy syndrome by maternal serum markers analysis in middle pregnancy.Among 1976 cases,3 cases were DS,1 case was 18-trisomy syndrome,9 cases were detected birth defects but their chromsonic normal.Con-clusion：Combined tests of AFP and F-βHCG using DELFIA in middle pregnancy may be effective and easily-accepted technique to screen congenital defect especially DS and 18-trisomy syndrome.

关 键 词：时间分辨荧光免疫分析技术 唐氏综合征 18-三体综合征 产前筛查 产前诊断 

分 类 号：R71[医药卫生—妇产科学]