电离辐射诱导T淋巴细胞的差异蛋白质组学研究  

Differential Proteomic Study of Human T Lymphocytic Leukemia Protein Induced by Ionizing Radiation

作　　者：梁峰[1] 石莹[2] 张萱[3] 刘忠英[1] 刘志强[2] 

机构地区：[1]吉林大学药学院,长春130021 [2]中国科学院长春应用化学研究所,长春质谱中心,长春130022 [3]吉林大学公共卫生学院,长春130021

出　　处：《高等学校化学学报》2010年第6期1143-1147,共5页Chemical Journal of Chinese Universities

基　　金：国家自然科学基金(批准号:20675079,20873137,30672600)资助

摘　　要：应用电离辐射诱导人类T淋巴细胞白血病Jurkat细胞株,再经二维凝胶电泳分离不同照射剂量的细胞总蛋白,研究电离辐射诱导的人类T淋巴细胞白血病细胞蛋白的差异表达.应用基质辅助激光解吸电离飞行时间质谱(MALDI-TOF-MS)进行处理,得到肽质量指纹(PMF)图谱,确认6个差异表达蛋白点.Two-dimensional electrophoresis and matrix-assisted laser desorption/ionization time-of-flight mass spectrorometry（MALDI-TOF-MS） have been acknowledged as the efficient methods in proteomics studies.In this report,the total proteins of human T lymphocytic leukemia（Jurkat cells） treated by ionizing radiation were separated with two-dimensional electrophoresis.The differential expression of cellular proteins induced by ionizing radiation was analyzed by PDQUEST software.Six differential protein spots were identified by MALDI-TOF-MS,including Lamin B1,heterogeneous nuclear ribonucleoprotein,p47 protein isoform a,alternative splicing factor 1,prohibitin,and 6-phosphogluconolactonase.The results may help researchers better understand the underlying mechanism involved in cellular protein alteration induced by ionizing radiation.

关 键 词：蛋白质组 二维凝胶电泳 基质辅助激光解吸电离飞行时间质谱 JURKAT细胞 

分 类 号：O657.63[理学—分析化学]