Htra1mRNA在PCOS大鼠卵巢颗粒细胞中的表达及意义  

The expression and importance of the Htra1 in the ovary Granulosa cells of PCOS rat model

作　　者：温宜萍[1] 钟刚[1] 

机构地区：[1]华中科技大学同济医学院附属同济医院,武汉430030

出　　处：《中国优生与遗传杂志》2010年第7期10-12,共3页Chinese Journal of Birth Health & Heredity

摘　　要：目的探讨Htra1 mRNA在PCOS大鼠卵巢颗粒细胞中的表达情况。方法皮下注射DHEA建立大鼠PCOS模型,并验证用RT-PCR方法及凝胶成像系统检测分析两组大鼠卵巢中Htra1的表达情况。结果与对照组比较,PCOS模型组血清雄激素(T),空腹血糖(FBG),空腹胰岛素(FINS)显著升高(P<0.05)。卵巢切片HE染色显示:模型组卵巢有较多的囊状扩张卵泡,而发育阶段卵泡与颗粒细胞层数明显减少,未见黄体。对照组可见不同发育阶段的卵泡及多个黄体;模型组卵巢组织Htra1 mRNA的表达显著低于对照组(P<0.05)。结论高雄激素血症和高胰岛素血症可能导致Htra1表达减弱,Htra1的低表达可能与多囊卵巢综合征(PCOS)卵泡发育成熟障碍及不排卵有关。Objective：To investigate the expression of Htra1 mRNA in ovary granulocytes of the PCOS rats.Methods：To Establish the PCOS rat model by subcutaneous injecting DHEA,and prove the model;The expression of Htra1 mRNA in the ovary granulose cells of the two groups were measured and evaluated by semi-quantitative RT-PCR and gel image analysis system.Results：The serum level of testosterone（T）,fast glucose（FBG）and fasting insulin （FINS） concentration were significantly higher in experimental group than those in control group（P〈0.05）.The HE stain of ovary slices showed that in the model groups,the number of developing follicles and granulose cells were diminished significantly,and the corpus lutea disappeared.Many developing follicles and corpus lutea were showed in the control groups;Compared with the control groups,the gel image analysis system demonstrated that the transcription of Htra1 mRNA were decreased significantly in PCOS groups（P〈0.05）.Conclusion：The hyperandrogen and the hyperinsulinemia may possible result in the low expression of Htra1 in the granulosa cells,which may have a close relationship with the folliclar maturation arrest and the anovlation in PCOS rats.

关 键 词：HTRA1 多囊卵巢综合征 卵泡发育 大鼠 

分 类 号：R711.75[医药卫生—妇产科学]