白术规范化种植中农药多菌灵安全使用标准的研究  

Safe use of carbendazim in GAP of Atractylodes macrocephala

作　　者：林建[1] 魏厚道[1] 王天玉[1] 吴加伦[1] 薛健[2] 

机构地区：[1]浙江大学农药与环境毒理研究所,浙江杭州310029 [2]中国医学科学院北京协和医学院药用植物研究所,北京100193

出　　处：《中国中药杂志》2010年第13期1674-1678,共5页China Journal of Chinese Materia Medica

基　　金：国家"重大新药创制"科技重大专项(2009ZX09502-027);浙江省社会发展项目(2008C23061)

摘　　要：目的:研究了多菌灵在白术根、土壤中的残留消解动态,并对其安全使用标准进行了讨论。方法:采用甲醇提取,液液分配净化,高效液相色谱分析。结果:不同添加浓度下回收率为86.1%～98.3%,RSD 1.0%～6.5%,满足农药残留检测要求。田间试验分别用推荐剂量(0.675 kg a.i..hm-2)和1.5倍推荐剂量(1.000 kg a.i..hm-2)进行处理,2年两地结果表明,多菌灵50%WP在白术土壤中的半衰期为6.51～7.98 d,在根中残留趋势为先升后降,半衰期为4.51～6.50 d,初加工后干样多菌灵的残留量为0.042～0.433 mg.kg-1,明显高于加工前新鲜样品残留量。结论:如果多菌灵在白术及其土壤中的MRL(最大残留限量)值推荐为0.2 mg.kg-1,建议按照常规剂量(0.675 kg a.i..hm-2)每年喷施2次,白术上最后一次使用多菌灵距收获的安全间隔期可考虑暂定为21 d。Objective： To study the residue of in roots of Atractylodes macrocephalal and in soil.Method： Samples were extracted with methanol.The extracts were cleaned up by liquid-liquid extraction and detected by HPLC.Result： Repeatability and accuracy of the method was verified by fortified recovery at 0.01,0.05,0.1,0.2 mg·kg-1 levels.Average recovery were 86.1%-98.3% and RSD were 1.0%-6.5% in root and soil.A.macrocephala was treated with two dosage of carbendazim during growing.Results of field test showed that the half lives of carbendazim were 6.51-7.98 d in cultivated soil,4.51-6.50 d in roots,separately.After sample was preliminarily processed,the residue of dried samples was 0.042-0.433 mg·kg-1,higher than the fresh samples.Conclusion： If 0.2 mg·kg-1 is recommended as the MRL（maximum residues limited） of carbendazim in the roots of A.macrocephala,it is suggested that the dose of 0.675 kg a.i.·hm-2 carbendazim is sprayed twice a year,and carbendazim should not be used within 21 days before the harvest.

关 键 词：白术 多菌灵 消解 安全间隔期 

分 类 号：S435.672[农业科学—农业昆虫与害虫防治]