补肾通脉方对多囊卵巢综合征胰岛素抵抗的大鼠磷脂酰肌醇-3激酶表达的影响  

Effects of Bushen Tongmai recipe on expression of phosphatidylinositol-3-kinase in PCOS rats accompanying with insulin resistance

作　　者：黄冬梅[1] 李琼[1] 陆付耳[1] 谢阳[1] 徐丽君[1] 邹欣[1] 龚菂 

机构地区：[1]华中科技大学同济医学院附属同济医院中西医结合研究所,湖北武汉430030

出　　处：《中国中药杂志》2010年第13期1735-1739,共5页China Journal of Chinese Materia Medica

基　　金：国家自然科学基金项目(30600838)

摘　　要：目的:观察补肾通脉方对多囊卵巢综合征(polycystic ovarian syndrome,PCOS)胰岛素抵抗(insulin resistance,IR)的大鼠肝脏、脂肪、肌肉和卵巢组织中磷脂酰肌醇-3激酶(PI-3K)p85α亚基的mRNA及蛋白表达的影响。方法:23日龄雌性SD大鼠皮下注射硫酸普拉睾酮钠90 mg.kg-1.d-1,共20 d,联合高脂饮食喂养80 d建立IR的PCOS大鼠模型,成模大鼠随机分为模型组和治疗组,另设15只正常对照组。治疗组大鼠以补肾通脉方进行干预。光镜下观察大鼠排卵情况;葡萄糖氧化酶法测定空腹血糖(FBG);放射免疫法测定空腹血清胰岛素(Fins);RT-PCR检测PI-3K p85α的mRNA表达;免疫组化检测卵巢PI-3K p85α的蛋白表达。结果:与模型组比较,治疗组平均排卵数及排卵率明显增加(P<0.01),Fins浓度显著降低(P<0.01)。治疗组靶组织PI-3K p85α的mRNA和蛋白表达水平均较模型组明显增加(P<0.01或P<0.05)。结论:补肾通脉方具有改善IR和排卵障碍的作用,其机制可能与其上调伴IR的PCOS大鼠靶组织PI-3K p85α的mRNA和蛋白表达有关。Objective： To observe the effects of Bushen Tongmai recipe（BSTMR） on mRNA and protein expressions of phosphatidylinositol-3-kinase（PI-3K） p85α in hepatic,adipose,muscular and ovarian tissues in PCOS rats with insulin resistance（IR）.Method： Twenty-three-day-old female SD rats were injected subcutaneously with sodium prasterone sulfate（90 mg·kg-1·d-1） for 20 days,and fed with high-fat forage for 80 days to induce PCOS rats with IR.Then the rats were randomly divided into the model group and the treated group.Meanwhile,a group of fifteen rats of the same age was considered as the normal control group.The treated group were administered with BSTMR.The ovulation condition was examined by hematoxylin-eosin（HE） staining.Fasting blood glucose（FBG） was determined using glucose oxidase method.Serum fasting insulin（Fins） was determined by radioimmunoassay（RIA）.The mRNA level of PI-3K p85α was measured by reverse transcription polymerase chain reaction（RT-PCR）.Immunohistochemistry staining was wtilised to detect protein expression of PI-3K p85α in ovary.Result： Compared with the model group,the mean number of corpus luteum and the rate of ovulation in the treated group increased significantly（P0.01）.The level of Fins in the treated group was much lower than that in the model group（P0.01）.Both mRNA and protein expressions of PI-3K p85α in target tissues were up-regulated significantly in the treated group（P0.01 or P0.05）.Conclusion： BSTMR could improve IR and ovulation dysfunction in PCOS rats accompanying with IR and its molecular mechanisms might be closely related with the elevation of mRNA and protein levels of PI-3K p85α in target tissues of the model rats.

关 键 词：多囊卵巢综合征 胰岛素抵抗 补肾通脉方 磷脂酰肌醇-3激酶 

分 类 号：R285.5[医药卫生—中药学]