扶正化瘀方与黄芪汤合用对实验性肝硬化大鼠的治疗作用  

Effect of Fuzheng Huayu recipe and Huangqi tang on DMN-induced experimental liver cirrhosis in rats

作　　者：王骆冰[1] 闫秀川[1] 曾贞[1] 吕靖[1] 刘平[1,2] 刘成海[1,2] 

机构地区：[1]上海中医药大学附属曙光医院肝病研究所,上海201203 [2]上海高校中医内科学E-研究院,上海201203

出　　处：《中国中药杂志》2010年第13期1740-1744,共5页China Journal of Chinese Materia Medica

基　　金：国家重点基础研究发展计划(973)项目(2006CB504801);国家自然科学基金项目(30772869);上海市优秀学科带头人计划项目(08XD14041);上海市教育委员会E-研究院建设计划项目(E0308);上海市教委创新团队建设项目

摘　　要：目的:观察扶正化瘀方与黄芪汤合用对实验性肝硬化大鼠的治疗作用,探讨补虚中药对肝硬化的治疗作用特点。方法:二甲基亚硝胺(DMN)腹腔注射4周制备肝硬化大鼠模型。成模后模型大鼠分别给予扶正化瘀方、黄芪汤、扶正化瘀方合黄芪汤灌胃2周。观察大鼠体重、肝重、脾重;HE染色观察肝组织炎症病理;天狼星红染色观察肝组织胶原沉积;试剂盒检测大鼠血清肝功能(ALT,Alb,T.Bil)、肝组织SOD活性及MDA含量;盐酸水解法测定肝组织Hyp含量;免疫组化染色观察肝组织α-SMA表达;Western blot检测肝组织α-SMA蛋白表达。结果:与正常大鼠相比,模型大鼠血清ALT,T.Bil明显升高,Alb含量显著降低;肝组织SOD活性下降,MDA和Hyp含量显著升高;肝组织炎症病变和胶原沉积明显,α-SMA表达显著增加。黄芪汤、扶正化瘀方及两方合用均可显著改善模型大鼠血清肝功能,改善肝脏脂质过氧化,降低肝组织Hyp含量,减轻肝组织炎症和胶原沉积,抑制α-SMA表达。在改善肝脏胶原沉积与抑制α-SMA表达方面,两方合用优于单用。结论:扶正化瘀方、黄芪汤、扶正化瘀方合黄芪汤均可改善DMN模型大鼠肝纤维化,两方合用较之各单方组抑制胶原沉积明显,其部分机制在于两方合用可更好抑制肝星状细胞活化。Objective： To investigate the effects of Fuzheng Huayu recipe and Huangqi tang on DMN-induced experimental liver cirrhosis in rats and explore the therapeutic characteristics of Buxu herbals on liver cirrhosis.Method： Liver cirrhosis in rats was induced by intraperitoneally injection of DMN for 4 weeks.Cirrhotic rats were randomly divided into 4 groups： model group,and Fuzheng Huayu recipe group,Huangqi tang group,Fuzheng Huayu recipe combined with Huangqi Tang group.The rats in treatment groups were orally administered with Fuzheng Huayu recipe,Huangqi tang,Fuzheng Huayu recipe combined with Huangqi tang（1∶1）,respectively.Normal and model control rats were given the equivalent normal saline.The body weight,liver weight and spleen weight were observed when rats were sacrificed.Liver histology was examined by HE staining and Sirius red staining.The liver function parameters including ALT,T.Bil and Alb were determined.The SOD activity and MDA content in liver tissues were also measured.Hepatic hydroxyproline（Hyp） content was determined by Jamall＇s method.The expression of α-SMA was determined by both immunohistochemistry staining and western blot method.Result： Compared with normal rats,the serum ALT and T.Bil levels in model rats increased obviously,by contrast,the serum Alb level decreased.There was a significant decline of SOD activity in model rat liver tissue,while the content of MDA and Hyp increased remarkably.A severe deterioration of liver architecture,infiltration of inflammatory cells and deposition of collagen were observed in model rat liver tissue.The expression of α-SMA also increased significantly.Compared with model rats,the liver function,lipid peroxidation parameters,Hyp content and liver histology were all improved in the 3 treatment groups.The combined group is better than any single-use group in decreasing collagen deposition and expression of α-SMA.Conclusion： Fuzheng Huayu recipe,Huangqi tang,Fuzheng Huayu recipe combined with Huangqi tang can attenuate liver fibrosi

关 键 词：肝硬化 肝星状细胞 胶原 扶正化瘀方 黄芪汤 

分 类 号：R285.5[医药卫生—中药学]