脆弱类杆菌脂多糖(LPS)对正常人外周血单个核细胞分泌IL-10及凋亡的影响  

Effect of LPS from Bacteroides Fragilis on Production of IL-10 and Apoptosis of Normal human PBMC

作　　者：周捷[1] 黄晓元[1] 

机构地区：[1]中南大学湘雅医院烧伤整形科,湖南长沙410008

出　　处：《中国现代医学杂志》2010年第9期1324-1327,共4页China Journal of Modern Medicine

基　　金：国家自然科学基金(No:30471785)

摘　　要：目的通过研究脆弱类杆菌LPS对正常人外周血单个核细胞(PBMC)分泌IL-10及凋亡的影响,探讨脆弱类杆菌感染的机制,为临床上防治厌氧菌感染提供科学的理论依据。方法采用脆弱类杆菌临床分离菌和标准菌株(NCTC9343)提取的LPS,以不同浓度梯度作用于正常人外周血单个核细胞,24h后收集培养细胞上清液,运用ELISA法检测细胞上清液中IL-10的含量变化,溴乙啶-吖啶橙染色检测凋亡百分率。结果脆弱类杆菌LPS对正常人PBMCIL-10分泌有显著刺激作用,并能诱导其凋亡(P<0.01)。脆弱类杆菌临床分离菌与标准菌株LPS对正常人PBMC分泌IL-10及凋亡具有相同效应,两组之间无明显差异(P>0.05)。结论脆弱类杆菌LPS对正常人PBMIL-10的分泌有刺激作用,并能诱导其凋亡。【Objective】To investigate the effect of LPS from Bacteroides Fragilis on production of IL-10 and Apoptosis of normal human PBMC,and explore the mechanism of Bacteroides Fragilis infection.Moreover,our experimental research will provide theory basis for prevention and treatment of clinical anaerobe infection.【Methods】 Different concentrations of LPS from Bacteroides Fragilis clinical isolate strain and standard strain NCTC9343 challenged PBMC from normal human,supernatant of cultured PBMC was collected after 24 hours.Changes of IL-10 in the supernatant were measured by ELISA and the ratio of apoptosis cell of PBMC was counted.【Results】LPS of Bacteroides Fragilis increased the secretion of IL-10 from normal human PBMC significantly（P 0.01）,and the effect of increase was concentration-depended,and LPS of Bacteroides Fragilis has a high potential to promote the apoptosis of PBMC.Meanwhile,The effect of LPS of Bacteroides Fragilis clinical isolate strain and standard strain on the secretion of IL-10 from normal human PBMC was identical.But the effect of two group of Bacteroides Fragilis LPS had no significance.【Conclusion】LPS of Bacteroides Fragilis stimulate the secretion of IL-10 from normal human PBMC,whereas promote the apoptosis.

关 键 词：脆弱类杆菌 LPS PBMC IL-10 凋亡 免疫抑制 感染 

分 类 号：R63[医药卫生—外科学]