姜黄素对THP-1细胞Toll样受体4信号传导通路中相关因子mRNA水平的影响  

Effects of curcumin on mRNA expression of cytokines related to Toll-like receptor 4 signaling in THP-1 cells

作　　者：彭立新[1] 李新宇[1] 宋莎莎[1] 王永芳[1] 徐兰芳[1] 

机构地区：[1]中国医学科学院北京协和医学院皮肤病研究所,南京210042

出　　处：《中华皮肤科杂志》2010年第7期493-496,共4页Chinese Journal of Dermatology

摘　　要：目的 研究姜黄素对人单核细胞株THP-1 Toll样受体4（TLR4）信号传导通路中相关因子mRNA水平的影响.方法 用不同浓度姜黄素（50、25、12.5 mg/L）预处理THP-1细胞12 h,0.043 mg/L地塞米松处理组作为阳性对照,继而用1 mg/L脂多糖（LPS）刺激诱导细胞4 h,以不做处理的THP-1作为阴性对照,仅用1 mg/L LPS刺激的THP-1作为LPS刺激组.提取细胞总RNA,用RT-PCR方法检测TLR4信号传导通路中TNF受体相关因子（TRAF）6、IL-1受体相关激酶（IRAK1）、核因子κB（NF-κB）mRNA的表达情况.结果 未经处理的THP-1细胞经LPS刺激4 h,可以显著提高TRAF6、IRAK1、NFκB mRNA的表达,与阴性对照组比较,t值分别为38.69、39.13、23.99（P〈0.01）. 50、25 mg/L姜黄素可以明显抑制THP-1细胞经LPS诱导后TRAF6、IRAK1、NF-κB mRNA的表达（P值均〈0.01）,抑制率均在50%以上.结论 一定浓度的姜黄素可以抑制LPS诱导后THP-1细胞TRAF6、IRAK1、NF-κB mRNA的表达,表明姜黄素对TLR4信号传导通路中相关因子mRNA水平有调节作用.Objective To investigate the effects of curcumin on mRNA expression of cytokines related to Toll-like receptor （TLR） 4 signaling in THP-1 cells.Methods After pretreatment with different concentrations （50,25,12.5 mg/L） of curcumin or dexamethasone for 12 hours,THP-1 cells were stimulated by lipopolysaccharide （LPS.1 mg/L） for 4 hours followed by the collection of cells.Then total RNA was isolated from these cells and subjected to reverse transcription-polymerase chain reaction （RT-PCR） for the detection of mRNA expression of tumor necrosis factor receptor-associated factor 6 （TRAF6）,interleukin-1 receptorassociated kinase （IRAK1） and nuclear factor （NF）-κB.THP-1 cells without pretreatment or stimulation served as negative control,and those only stimulated with LPS served as LPS group.Results After stimulation with LPS （1 mg/L） for 4 hours,the mRNA expressions of TRAF6,IRAK1 and NF-κB were significantly upregulated in THP-1 cells compared with negative control cells （f=38.69,39.13,23.99,all P〈0.01）.Curcumin of 50 mg/L and 25 mg/L significantly inhibited the mRNA expressions of TRAF6.IRAK 1 and NF-κB upregulated by LPS with an inhibition rate of more than 50% （all P〈0.0 1）.Conclusions Certain concentrations of curcumin can inhibit the mRNA expressions of TRAF6.IRAK1 and NF-κB.which demonstrates the regulatory effect of curcumin on the mRNA expressions of TLR4 signaling pathway-associated cytokines.

关 键 词：姜黄素 TNF受体相关因子6 白细胞介素1受体相关激酶类 NF—κB 细胞系 

分 类 号：R285.5[医药卫生—中药学]