毒热平注射液体外对小鼠T细胞功能及杀伤FM1感染靶细胞功能的影响  

Effect of Dureping Injection on T-cells Function and Their Function in Killing FM1 Infected Mφin Vitro

作　　者：景姗[1] 顾立刚[2] 周芸[2] 李澎涛[1] 

机构地区：[1]北京东直门医院消化内科,北京100700 [2]北京中医药大学中医药抗病毒教育部重点实验室

出　　处：《中国中西医结合杂志》2010年第7期729-732,共4页Chinese Journal of Integrated Traditional and Western Medicine

基　　金：国家自然科学基金资助项目(No.30772872)

摘　　要：目的观察毒热平注射液体外对小鼠T细胞功能及杀伤流感病毒亚甲型鼠肺适应株(FM1)感染靶细胞的影响。方法用MTT法及双抗体夹心ELISA法检测毒热平注射液低、中、高(2.1、8.5、17.0μg/mL)剂量对正常小鼠、感染流感病毒亚甲型鼠肺适应株(FM1)的脾细胞数目、产生干扰素-γ(IFN-γ)、白介素-10(IL-10)、及T细胞杀伤FM1感染巨噬细胞Ana-1功能的影响。结果毒热平注射液体外能抑制刀豆蛋白A(Con A)诱导的正常小鼠脾细胞的增殖,抑制Th2型细胞因子IL-10的产生,促进Th1型细胞因子IFN-γ的产生;能保护FM1感染的小鼠脾细胞,并抑制其产生Th2型细胞因子IL-10,而使Th1型细胞因子IFN-γ水平保持在一定的范围内;还能直接增强T细胞对FM1感染的靶细胞(Ana-1)的杀伤能力(P<0.05,P<0.01)。结论毒热平注射液体外作用于小鼠T细胞,可增强有利于抗FM1感染的免疫应答。Objective To investigate the effect of Dureping Injection （DRP） on the T-cells function of mice and the function of T-cells in killing Mφ infected by influenza virus subtype A mice-lung adaptive strain FM1 in vitro. Methods Number of splenic normal and FM1 infected T-cells in mice were measured by MTT and double-antibody sandwich ELISA, after being treated with DRP at different concentrations （2.1,8.5 and 17.0 μg/mL）, and the effect of DRP on interferon-γ （IFN-γ） and interleukin-10 （IL-10） production as well as on splenic T-cell killing FM1 infected Mφ/Ana-1 function were detected. Results DRP inhibited the multiplication of normal spleen T cells inducead by concanavalin A in vitro, suppressed Th2 cell factor IL-10 production, and maintained Thl cell factor IFN-γ at a definite level, moreover, it directly enhanced the power of T-cells in killing FM1 infected Mφ （ P 〈 0.05, P 〈 0.01 ）. Conclusion DRP could act on mice T-cells to enhance the immune response for antiinfluenza viral FM1 in vitro.

关 键 词：毒热平注射液 流感病毒亚甲型鼠肺适应株(FM1) T细胞 体外实验 

分 类 号：R285.5[医药卫生—中药学]