福氏痢疾杆菌2b血清型菌株基因组中SfII和SfX前噬菌体整合位点及排列方式研究  

Study on the integration site and arrangement of SfII and SfX prophages in Shigella flexneri serotype 2b strains

作　　者：王艺婷[1] 孙强正[1] 刘凯[1] 赵爱兰[1] 王艳[1] 金东[1] 李振军[1] 景怀琦[1] 叶长芸[1] 徐建国[1] 

机构地区：[1]中国疾病预防控制中心传染病预防控制所传染病预防控制国家重点实验室,北京102206

出　　处：《中华流行病学杂志》2010年第7期800-803,共4页Chinese Journal of Epidemiology

基　　金：北京市优秀博士学位论文指导教师科技项目（YB20098450101）;传染病预防控制国家重点实验室自主研究重点项目（2008DFA31830）

摘　　要：目的 研究福氏痢疾杆菌2b血清型菌株中SfII和SfX前噬菌体整合位点及排列方式.方法 根据2b血清型菌株基因组中SFII和SfX前噬菌体可能的整合位点及排列方式,设计引物,对50株2b血清型菌株进行PCR扩增,并对扩增产物测序,根据扩增和测序结果判断噬菌体的整合位点和排列方式.结果 在2b血清型菌株中,SfII和SfX前噬菌体前后相连,整合于宿主菌染色体proA和yaiC基因间的thrW tRNA基因上;以SfII在前,SfX在后,或SfX在前,SfII在后两种方式排列;而以后一种方式为主,在共50株检测菌株中,有49株为此方式.结论 福氏痢疾杆菌血清型相关噬菌体的整合位点及排列方式,对研究福氏痢疾杆菌血清型转换及血清型相关噬菌体的整合机制具有重要意义.Objective To study the integration site and arrangement of SfII and SfX prophages in Shigella flexneri serotype 2b strains. Methods A series of primers were designed based on potential integration site of SfII and SfX prophages in Shigella flexneri serotype 2b strains, and PCR were performed for 50 serotype 2b strains to amplify special genes located in host and prophages. PCR products were sequenced to identify integration sites and arrangement of SfII and SfX. Results In all the serotype 2b strains, prophage SfII and SfX were adjacent to each other, and integrated into the thrW tRNA gene of the host, which were located between genes proA and yaiC of host. Prophage SfX was located immediately upstream of prophage SfII in all the detected 50 serotype 2b strains exception for strain 51251. Conclusion This was the first report on the integration site and arrangement of serotype-converting prophages SfII and SfX in Shigella flexneri 2b strains.

关 键 词：福氏痢疾杆菌 血清型 前噬菌体 整合位点 排列方式 

分 类 号：R378[医药卫生—病原生物学]