SDF-1 3'A和CCR5△32在评估扩张型心肌病预后中的意义  

Significance of SDF-1 3′A and CCR5△32 in evaluating the prognosis of patients with dilated cardiomyopathy

作　　者：李燕燕[1] 赵永静[1] 孙改河[1] 黄凯达[1] 任春锋[1] 沈燕[1] 

机构地区：[1]郑州大学第一附属医院检验科,河南郑州450052

出　　处：《中国现代医学杂志》2010年第10期1470-1473,共4页China Journal of Modern Medicine

基　　金：河南省科技攻关项目(No:0496060902);河南省教育厅自然科学基金项目(No:2009A320034)

摘　　要：目的研究SDF-13'A和CCR5△32在评估扩张型心肌病患者预后中的意义。方法设置正常对照组和扩张型心肌病(DCM)组,利用PCR-RFLP和PCR方法检测SDF-1和CCR5的基因多态性,分析SDF-1和CCR5的突变频率,并比较此突变频率与心功能分级、左室射血分数(LVEF)和心律失常间的关系。结果①在心功能Ⅰ～Ⅲ级和心功能Ⅳ级的DCM患者中,SDF-13'A突变率分别为0.35和0.18,差异有显著性(P<0.05);②在LVEF>35%与LVEF<35%的DCM患者中,SDF-13'A突变率分别为0.40和0.13,差异有显著性(P<0.05);③DCM患者及健康人中未现CCR5△32突变。结论 SDF-13'A突变可用于DCM患者的预后评估,提示在DCM中SDF-1可能参与心功能损伤的发生过程。[Objective]To research the significance of SDF-1 3’A and CCR532 in evaluating the prognosis of patients with dilated cardiomyopathy（DCM）.[Methods]The research objects were divided into two groups （the normal control group and the DCM group）. The Gene polymorphism of SDF-1 and CCR5 were detected by using PCRRFLP and PCR methods. The mutation frequencies of SDF-1 and CCR5 were analyzed, and the relations between these mutation frequencies and the grading of cardiac function, left ventricular ejection fraction （LVEF） and cardiac arrhythmia has been studied. [Results] The SDF-1 3＇A mutation frequencies of the DCM patients with cardiac function Ⅰ～Ⅲ level and the Ⅳ level were respectively 0.35 and 0.18, so there was a prominent difference （P 0.05）. The SDF-1 3＇A mutation frequencies of DCM patients with LVEF 35% and LVEF 35% were 0.40 and 0.13 respectively, and there was a significant difference （P0.05）. There were no mutations in the CCR5 32 among DCM patients and healthy people.[Conclusion]SDF-1 3＇A mutation can be used to evaluate the prognosis of patients with DCM. It can suggest the occurrence process of cardiac function injury in DCM with which the SDF-1 may be concerned.

关 键 词：基因多态性 SDF-1 CCR5 突变 扩张型心肌病 

分 类 号：R446.6[医药卫生—诊断学]