NCS-382对异氟烷催眠镇痛作用的影响  

Effects of NCS-382 on hypnotic and analgesic effects of isoflurane

作　　者：周慧轩[1] 徐海虹[1] 杨龙[1] 丁钦[1] 沈杨[1] 曹春龙[1] 周美艳[1] 张洋[2] 戴体俊[3] 

机构地区：[1]徐州医学院麻醉学院,221002 [2]徐州医学院麻醉学院麻醉生理学教研室,221002 [3]徐州医学院麻醉学院麻醉药理学教研室,221002

出　　处：《上海医学》2010年第4期319-321,共3页Shanghai Medical Journal

基　　金：国家自然科学基金资助项目(30872432;30471657;39970715);江苏省自然科学基金资助项目(BK2001143);2009年江苏省高等学校大学生实践创新训练计划项目;徐州医学院2009年研究生科技创新计划(XYCX200916)

摘　　要：目的探讨NCS-382(γ-羟基丁酸受体阻断剂)对异氟烷(Iso)催眠、镇痛作用的影响。方法催醒实验中,建立小鼠Ios催眠模型,分为对照组和不同浓度NCS-382组,观察侧脑室注射NCS-382对小鼠睡眠时间(ST)的影响;热板、扭体实验中,分清醒组和Iso镇痛组,每组再分为对照亚组和不同浓度NCS-382亚组,观察鞘内注射NCS-382对小鼠热板法痛阈(HTTP)和扭体次数的影响。结果催醒实验中,侧脑室注射115、23、4.6、2.3、1.15μgNCS-382能够缩短异氟烷催眠小鼠的ST(P值均<0.01),0.92、0.184、0.0368μgNCS-382对异氟烷催眠小鼠的ST影响不大;热板、扭体实验中,115、4.6、0.184μgNCS-382对清醒小鼠和异氟烷镇痛小鼠的HTTP、扭体次数均无明显影响(P值均>0.05)。结论γ-羟基丁酸受体可能是Iso催眠作用的靶位之一,而非镇痛作用的主要靶位。Objective To investigate the effect of NCS-382 on the hypnotic and analgesic effects of isoflurane.Methods The mouse model of hypnosis was established and the model mice were divided into the control group and groups injected with different doses of NCS-382 （blocker of gamma hydroxybutyrate receptor）.The effect of intrathecal （i.t.） injection of NCS-382 on the sleeping time （ST） was evaluated.In hot-plate test and acetic acid-induced writhing test,the mice were divided into a conscious group and an analgesic group;the two groups were further divided into control group and groups injected with different doses of NCS-382.The effects of intrathecal （i.t.） injection of NCS-382 on the pain threshold in hot-plate test （HPPT） and writhing times were assessed.Results Injection of 115,23,4.6,2.3,and 1.15 μg NCS-382 （i.c.v.） decreased ST of the mice treated with isoflurane （P0.01）,while 0.92,0.184,and 0.036 8 μg NCS-382 had no significant effect on ST of mice （P0.05）.Injection of 115,4.6,and 0.184 μg NCS-382 （i.t.） did not affect the HPPT and writhing times of conscious mice and the mice treated with isoflurane （P0.05）.Conclusion GHB receptor may be one of the important targets for the hypnotic effects of isoflurane,and it may not be the main target for the analgesic effects.（Shanghai Med J,2010,33：319-321）

关 键 词：Γ-羟基丁酸受体 NCS-382 异氟烷 催眠 镇痛 

分 类 号：R614[医药卫生—麻醉学]