5-羟色胺2A受体阻滞剂保护糖尿病大鼠阴茎勃起功能作用的研究  

Protective effects of sarpogrelate hydrochloride on erectile function of diabetic rats

作　　者：陈凌[1] 何兰杰[1] 李国徽[2] 

机构地区：[1]宁夏医科大学附属医院,银川750004 [2]宁夏医科大学附属银川市中医医院

出　　处：《陕西医学杂志》2010年第7期771-775,779,共6页Shaanxi Medical Journal

基　　金：宁夏回族自治区自然科学基金(NZ0674)

摘　　要：目的:探讨5-羟色胺2A(5-HT2A)受体阻滞剂(盐酸沙格雷酯)对糖尿病大鼠阴茎勃起功能的影响及其作用机制。方法:设立正常对照组(NC组)、糖尿病组(DM组)、盐酸沙格雷酯治疗组(SH组)、西地那非治疗组(WG组)。SH组大鼠给予盐酸沙格雷酯100mg/(kg.d)灌胃,连续给药12周,WG组大鼠在进行阿朴吗啡(APO)诱导阴茎勃起实验前3d给予西地那非20mg/(kg.d)。在第0、4、8、12周进行APO试验;检测各组血清NO、5-HT、ET-1含量,计算NO/ET-1比值;免疫组化法检测阴茎海绵体内皮型一氧化氮合酶(eNOS)表达水平;光镜、透射电镜下观察各组大鼠阴茎组织病变情况。结果:药物干预第4、8、12周时,与DM组比较,SH组大鼠阴茎勃起率、勃起次数均有明显的增加;与WG组相比,SH组早期勃起率及勃起次数均低于WG组,中期上述指标与WG组接近,后期勃起率、勃起次数均高于WG组,但无统计学差异。与DM组相比,SH组大鼠阴茎组织eNOS蛋白表达水平上调,血清5-HT浓度下降,NO浓度上升,NO/ET-1比值上升,阴茎海绵体病理损伤明显减轻。结论:5-HT2A受体阻滞对糖尿病大鼠勃起功能有保护作用。5-HT及5-HT2A受体在糖尿病性ED的发生中具有重要作用。Objective：To approach the protective effect of sarpogrelate hydrochloride on erectile function of diabetic rats.Methods：SD rats were divided into four groups：（NC） normal controls group;（DM）diabetic rats group;（SH） sarpogrelate hydrochloride-treated group[100mg/（kg·d）];（WG）sildenafil-treated group[20mg/（kg·d） for 3 days just before APO test）].The administration was kept on for 12 weeks.The APO test was used to evaluate the erectile function at 4,8,12 week.Levels of blood NO,ET-1,5-HT were measured.Expression of eNOS was determinated by immunohistochemistry.The pathological changes of penis will be observed by using light microscope and electronmicroscope.Results：At 4,8,12 week,erection rate and frequency in SH group were higher than that in DM group;Compared with WG group,two items in SH group were lower at 4 week,and were higher at 12 week.Compared with DM group,expression of eNOS in penis were up-regulated,5-HT levers was lower and NO levers was higher,ratios between NO and ET-1 was higher in SH group.The pathologic changes in SH group were improved.Conclusion：Sarpogrelate hydrochloride can ameliorate diabetic erectile function.5-HT and 5-HT2AR play an important role in the pathogenesis of DED.

关 键 词：糖尿病 阳萎 @5-羟色胺2A受体阻滞剂 @内皮型一氧化氮合酶 大鼠 

分 类 号：R339.21[医药卫生—人体生理学]