秦川牛肝过氧化氢酶提取因素及优化的研究  

Optimization of Factors for Catalase Extraction from Qinchuan Cattle Liver

作　　者：马志科[1] 昝林森[1] 马丽[1] 

机构地区：[1]西北农林科技大学动物科技学院,陕西杨凌712100

出　　处：《西北农业学报》2010年第6期52-54,共3页Acta Agriculturae Boreali-occidentalis Sinica

基　　金：国家自然科学基金项目(30871788)资助

摘　　要：以秦川牛肝为原料采用缓冲液浸提法提取过氧化氢酶,先选择影响提取的主要因素(浸提pH、浸提温度和浸提时间)分别作为单因素进行梯度试验,确定其合适范围;再通过正交试验确定牛肝过氧化氢酶提取条件的优化组合。结果表明,从秦川牛肝提取过氧化氢酶最佳条件为浸提pH 7.0、浸提温度30℃、浸提时间10 h。Catalase from Qinchuan cattle liver was studied by buffer extraction. Firstly, single factor tests with different gradients of pH, temperature, and extraction time were carried on independently to choose the main factors and the scope of each single factor. Then the orthogonal trial of L9 （3 3） was designed to acquire the most suitable combination conditions for catalse extraction. The results showed that the optimum extraction conditions of catalase from Qinchuan cattle liver are to extract at 30℃ for 10 h, pH 7.0.

关 键 词：秦川牛肝 过氧化氢酶 提取因素 

分 类 号：S823[农业科学—畜牧学]