苹果抗斑点落叶病基因的一个RAPD标记的SCAR转换  

Development of a SCAR Marker Linked to Apple Spot Resistance Gene from a RAPD Marker

作　　者：祁楠[1] 万怡震[1] 高华[1] 樊红科[1] 赵政阳[1] 

机构地区：[1]西北农林科技大学园艺学院,陕西杨凌712100

出　　处：《西北农业学报》2010年第6期106-109,共4页Acta Agriculturae Boreali-occidentalis Sinica

基　　金：国家现代农业技术体系专项(nycytx-08-01-03);国家科技支撑计划(2006BAD01A1704-8);陕西省"13115"科技创新工程(2008ZDGC-01)

摘　　要：将苹果抗斑点落叶病相关基因的一个RAPD标记成功转换为重复性和特异性更好的SCAR标记。由序列特点设计特异SCAR引物,并用所设计引物对两亲本和抗病、感病基因池及F1代单株进行PCR扩增。结果表明,秦冠×富士F1代群体在抗病后代个体中扩增出470 bp条带,而在感病后代个体中无此扩增条带,故将该标记命名为SCAR470。同时,分析分离群体的扩增,重复率为4.69%,表明该标记可以用于苹果斑点落叶病的分子鉴定。A RAPD marker of apple spot resistance gene was converted into a SCAR marker successfully. Specific primers were designed according to the nucleotide sequence and tested in parents, bulks and in individuals of F1 progeny. Results showed that the primer pair could amplify a apple-spot-resistance-specific band of 470 bp. This SCAR marker was named as SCAR470. Meanwhile, the recombinant frequency is 4. 69% based on amplification analysis in a segregation population. These results further confirmed the feasibility of this SCAR marker in marker-assisted selection （MAS） for apple spot.

关 键 词：苹果 斑点落叶病 RAPD标记 SCAR标记 

分 类 号：S436.611[农业科学—农业昆虫与害虫防治]