甘蓝Ogura胞质雄性不育基因的RAPD标记  

Genomic DNA Extraction and Preliminary Study on RAPD Markers Linked to Ogura Cytoplasm Male Sterile Gene in Cabbage

作　　者：王小艳[1] 张恩慧[1] 许忠民[1] 程永安[1] 许念芳[1] 马勇斌[1] 

机构地区：[1]西北农林科技大学园艺学院,陕西杨凌712100

出　　处：《西北农业学报》2010年第6期130-133,共4页Acta Agriculturae Boreali-occidentalis Sinica

基　　金：国家"十一.五"科技支撑计划资助项目(2008BADB1B02;2006BAD01A7-2-04);陕西省"13115"重大科技专项资助项目(2008ZDKG-03);农业部公益性行业(农业)科研专项经费项目(nyhyzx07-007)

摘　　要：以甘蓝Oguar胞质雄性不育系CMS451及其保持系为材料,优化甘蓝RAPD体系,并用RAPD标记分析了Oguar胞质不育基因。结果表明,适合甘蓝RAPD体系为,25μL中含有2.0 mmol/L MgCl2,0.2mmol/L dNTPs,0.56μmol/L pri mer,0.5 UTaqDNA聚合酶,20 ng DNA,2.5μL 10×buffer(剩余的用ddH2O补充);发现S20-495为不育基因的特异条带,在NCBI上比对此特异条带序列发现它与芜菁自交不亲和系SRK-46、SP11-46和SLG-46的基因全序列同源性达78%。In this study,using male sterile CMS451 and maintainer line of Ogura cytoplasm in cabbage as material. Optimized reaction system of RAPD,which was suited for cabbage,and analyzed male sterility gene of CMS451 by RAPD. The results showed that RAPD reaction system of cabbage was optimized：total volume was 25 uL,including 2.0 mmol/L MgCl2,0.2 mmol/L dNTPs,0. 56 umol/L primer. 0.5 U Taq DNA polymerase,20 ng DNA,2.5 uL 10×buffer,the rest was supplied with ddH2O. The specific band of sterile gene S20-495 was identified. Though NCBI Blast, S20-495 sequence has higher alignment score with Brassica self-incompatibility line SRK-46 ,SPll-46 ,SLG-46 gene sequence up to 78%.

关 键 词：甘蓝 胞质雄性不育 RAPD 标记 

分 类 号：S635.1[农业科学—蔬菜学]