灯盏花素阳离子脂质体的制备  

Preparation of breviscapine cationic liposomes

作　　者：董晓东[1] 邓英杰[1] 陈超[1] 曹永仓[1] 崔丽莉[1] 王晓宇[1] 

机构地区：[1]沈阳药科大学药学院,沈阳110016

出　　处：《中国新药杂志》2010年第12期1075-1079,共5页Chinese Journal of New Drugs

基　　金：国家自然科学基金(30672555)

摘　　要：目的:制备灯盏花素阳离子脂质体和普通脂质体并对其相关性质进行考察。方法:采用薄膜分散法制备灯盏花素脂质体;以包封率为指标,通过正交试验对处方进行优化。考察灯盏花素普通脂质体和阳离子脂质体的体外释放行为。结果:制备的灯盏花素阳离子脂质体性质稳定,包封率为(76.42±1.973)%,平均粒径为(186±35)nm,Zeta电位为(48.9±9.83)mV。灯盏花素普通脂质体和阳离子脂质体的释放过程的拟合方程分别为lnln[1/(1-Q)]=0.7797lnt-2.3187(r=0.9739)和lnln[1/(1-Q)]=0.3553lnt-3.197(r=0.9899)。结论:确定了最优处方,制备得到包封率较高且状态稳定的灯盏花素阳离子脂质体。Objective：To prepare breviscapine cationic liposomes and ordinary liposomes,optimize the formulation and investigate characteristics of the liposomes. Methods：The breviscapine liposomes were prepared by thin-film dispersion method. With the entrapment efficiencies as references,the formulation was optimized by the orthogonal design. The in vitro release profiles of the liposomes were determined. Results：The prepared cationic liposomes were stable with the mean size of （186±35） nm,the Zeta potential of （48.9±9.83） mV and the entrapment efficiencies （76.42±1.973）%. The fitting equations of the release profiles of both kinds of liposomes were lnln^[1/（1-Q）]=0.779 7ln^t-2.318 7（r=0.973 9） and lnln^[1/（1-Q）]=0.355 3ln^t-3.197（r=0.989 9）,relatively. Conclusions：We have optimized formulation and obtained the stable and uniform breviscapine cationic liposomes.

关 键 词：灯盏花素 阳离子脂质体 包封率 

分 类 号：R943.4[医药卫生—药剂学] R972[医药卫生—药学]