羊耳菊药材中4,5-O-二咖啡酰基奎宁酸的含量测定  

Determination of 4,5-O-dicaffeoyl-quinic acid content in Herba inulae cappae by HPLC

作　　者：何迅[1] 王爱民[1] 李勇军[1] 王永林[1] 迟明艳[1] 

机构地区：[1]贵阳医学院药学院,贵阳550004

出　　处：《中国新药杂志》2010年第12期1084-1086,共3页Chinese Journal of New Drugs

基　　金：国家"十一五"科技支撑计划子课题(2006BAI06A01-3);贵州省中药现代化科技产业研究开发专项基金(黔科合中药专字[2005]5039号)

摘　　要：目的:建立羊耳菊药材中4,5-O-二咖啡酰基奎宁酸的含量测定方法,为其质量控制提供科学依据。方法:采用HPLC直接测定,色谱柱Eclipse XDB C18柱,保护柱为SecurityGuard C18柱;流动相甲醇-0.1%磷酸(28∶72);检测波长为325nm。结果:测定了13批不同产地市售的羊耳菊药材,4,5-O-二咖啡酰基奎宁酸的含量在0.12%～0.55%之间。结论:该法方便、快速、准确、重复性好,可以控制该药材的质量。Objective：To establish the quality standard of Herba inulae cappae for determining the 4,5-O-dicaffeoyl-quinic acid with HPLC. Methods：The separation was performed on a Eclipse XDB C18 column with SecurityGuard C18 protective column. The mobile phase consisted of methanol and 0.1% phosphoric（28：72）,the detection wavelength was set at 325 nm. Results：The contents of 4,5-O-dicaffeoyl-quinic acid in 13 samples of Herba inulae cappae in market were in the ranges of 0.12%-0.55%. Conclusion：The method is simple,accurate and reproducible,and is suitable for the quality control of Herba inulae cappae.

关 键 词：羊耳菊 4 5-O-二咖啡酰基奎宁酸 高效液相色谱法 

分 类 号：R978.7[医药卫生—药品] R927.2[医药卫生—药学]