大鼠前包氏复合体区神经元的放电类型和对甘氨酸的反应  

DISCHARGE PATTERNS OF NEURONS IN PREBOTZINGER COMPLEX AND ITS RESPONSES TO GLYCINE IN RATS

作　　者：张翠英[1] 余微[1] 郑煜[1] 

机构地区：[1]华西医科大学生理学教研室

出　　处：《中国神经科学杂志》1999年第1期27-30,共4页

基　　金：国家教委科研基金

摘　　要：采用多管微电极细胞外记录和微电泳技术，分析了成年大鼠前包氏复合体（ｐｒｅ－Ｂｏｔｃ）区自发放电神经元的放电类型及它们对微电泳甘氨酸（Ｇｌｙ）及其拮抗剂士的宁（Ｓｔｒｙ）的反应。在４３只麻醉、自主呼吸大鼠的ｐｒｅ－Ｂｏｔｃ区共记录到１０４个自发放电神经元。其中呼吸相关神经元（ＲＲＮｓ）５４个，非呼吸相关神经元（ＮＲ－ＲＮｓ）５０个；ＲＲＮｓ包括吸气神经元（ＩＮｓ，ｎ＝２５）、呼气神经元（ＥＮｓ，ｎ＝１７）和跨时相神经元（ＰＳＮｓ，ｎ＝１２）三类。ＩＮｓ包括早吸气型（ｅａｒｌｙ－Ｉ，１１／２５个）神经元，全时程吸气型（Ｉ－ａｌ，１０／２５个）神经元和晚吸气型（ｌａｔｅ－Ｉ，４／２５个）神经元；ＥＮｓ包括全时程呼气型（Ｅ－ａｌ，７／１７个）神经元，早呼气型（ｅａｒｌｙ－Ｅ，６／１７个）神经元，晚呼气型（ｌａｔｅ－Ｅ，４／１７个）神经元；ＰＳＮｓ包括前吸气（ｐｒｅ－Ⅰ，８／１２个）神经元和吸气－呼气跨时相（Ｉ－Ｅ，４／１２个）神经元。微电泳Ｇｌｙ，使受试的２７个神经元中的２２个抑制，少数无反应（ｎ＝３）或兴奋（ｎ＝２），反应呈量效依赖关系。Ｓｔｒｙ使受试的２２个神经元中的８个兴奋，其余１４个无反应，兴奋反应呈量效依?The discharge patterns of neurons in preBotzinger complex (preBot c) and its responses to iontophoretic application of glycine (Gly) and strychnine (Stry) in adult rats were analyzed with multibarreled microelectrodes. One hundred and four units with stable spontaneous discharge were recorded in 43 rats. Among them 54 were respiratory related neurons (RRNs),while 50 were nonrespiratory related (NRRNs). Of the 54 RRNs,25,17,and 12 neurons were inspiratory(INs),expiratory (ENs),and phasespanning (PSNs),respectively. The INs could be subdivided into eraly (11/25),all(10/25) and late (4/25).The ENs could be subdivided into Eall (7/17),earlyE(6/17) and lateE (4/17). PSNs included preinpiratory(pre,8/12) and inspiratory expiratory phasespanning (IE,4/12) neurons.Gly inhibited most of the neurons tested (22/27),and elicited excitation(2/27) or no response (3/27) in a few of them.Eight out of the 22 neurons tested were activated by Stry,but others(14/22) were unaffected. The inhibitory effect of Gly and thestrychninerate excitatory effect of Stry showed a doseresponse dependent relationship.The inhibitory effect of Gly on 10 out of 12 neurons tested could be blocked by Stry. These results indicate that preBot c in adult rats is characterized by an inclusion of various types of respiratory neurons,especially the existence of PSNs.The relationship between distinct types of respiratory neurons in preBot c and their responsiveness to Gly and Stry were discussed.

关 键 词：前包氏复合体 神经元自发放电 甘氨酸 士的宁 大鼠 

分 类 号：R338.1[医药卫生—人体生理学] R338.8[医药卫生—基础医学]