格尔德霉素衍生物17-AAG对缺氧宫颈癌细胞凋亡的影响  

Effect of Geldanamycin Derivatives 17-AAG on Apoptosis of Uterine Cervix Cancer Cells in Hypoxia

作　　者：王德健[1] 徐世军[1] 李巧云[1] 

机构地区：[1]成都中医药大学药学院,四川成都610041

出　　处：《成都中医药大学学报》2010年第2期86-88,共3页Journal of Chengdu University of Traditional Chinese Medicine

摘　　要：目的:观察格尔德霉素衍生物17-AAG对氯化钴化学缺氧诱导的宫颈癌细胞凋亡的影响,并简单探讨可能的机制。方法:用含200μmol/LCoCl2的无血清RPMI 1640培养基模拟宫颈癌细胞缺氧微环境,West-ern Blot法检测缺氧诱导因子HIF-1α及其下游靶分子VEGF蛋白的表达,DAPI染色检测细胞的凋亡情况。结果:缺氧可诱导HIF-1α、VEGF蛋白的表达上调;但这种上调可被17-AAG所逆转,从而使宫颈癌细胞对缺氧的耐受能力明显降低,凋亡增加。结论:17-AAG通过对HIF-1α信号的干扰而具有一定的抗瘤活性。Objective：To explore the effect of geldanamycin derivatives 17-AAG on apoptosis of uterine cervix cancer cells in chemical hypoxia induced by CoCl2,and elucidate possible mechanism.Methods：RMPI 1640 medium containing 200μmol/LCoCl2 without serum was applied to mimic hypoxic microenvironment of uterine cervix cancer cells.The expression of HIF-1α and its downstrem target molecule VEGF protein was detected by Western blotting and Cell apoptosis detected by DAPI staining.Resluts：Upregulation of of HIF-1α and VEGF protein expression was induced by hypoxia,however this effect could be reversed by 17-AAG,therefore significantly decreased the tolerance of uterine cervix cancer cells to hypoxia,and promoted apoptosis.Conclusion：17-AAG showed some anti-cancer activity through intervention of HIF-1α signalling pathway.

关 键 词：宫颈癌 17-丙烯胺基-17-去甲氧基格尔德霉素 缺氧 凋亡 

分 类 号：R285.5[医药卫生—中药学] R966[医药卫生—中医学]