参附注射液对兔肺缺血再灌注细胞凋亡的保护作用及其机制探讨  

Investigate of the protective effect and mechanism of Shenfu injection on Lung ischemia-reperfusion injury

作　　者：蒲江涛[1] 廖斌[1] 刘伦旭[2] 戴天阳[1] 杨峥[1] 

机构地区：[1]泸州医学院附属医院胸心外科,四川泸州646000 [2]四川大学华西医院胸心外科,四川成都610041

出　　处：《细胞与分子免疫学杂志》2010年第8期816-817,共2页Chinese Journal of Cellular and Molecular Immunology

摘　　要：目的：观察参附注射液对肺缺血再灌注损的保护作用，并探讨其机制。方法：将30只兔随机分为假手术组（Sh组）、肺缺血再灌注组（IR组）和参附注射液组（SF组），每组10只，建立肺IR模型。Sh组于左肺门处以弯眼科镊引导7号指示线从下方绕过肺门，不予钳夹，于6h后取肺组织；IR组于留置指示线外侧用无创伤微血管钳钳夹肺门远心脏一端，阻断肺门45min，再开放6h后取肺组织；SF组于阻断肺门前30min耳缘静脉注射SF 10mL／kg。采用流式细胞术检测肺组织细胞凋亡率，采用原位杂交检测肺组织Bcl-2／Bax、Fas／FasL基因表达。结果：与Sh组比较，IR组、SF组肺组织细胞凋亡率及Bcl-2／Bax、Fas／FasL基因表达明显增加（P〈0．01）；与IR组比较，SF组肺组织细胞凋亡率及Bcl-2／Bax、Fas／FasL基因表达明显降低（P〈0．05）。结论：参附注射液对肺缺血再灌注损具有保护作用，与其抑制肺组织肺组织Bcl-2／Bax、Fas／FasL基因表达、减少细胞凋亡有关。AIM： To observe shenfu injection on lung ischemia-reperfusion damage of the protective effect and its mechanism. METHODS： 30 rabbits were randomly divided into sham operation group （Sh group）, lung ischemia-reperfusion group （IR group） and the SFI group （SF group）, n = 10, the establishment of lung IR model. Sh cutaneous group punishable by bending eye tweezers to guide instruction No. 7 line from the bottom around the hilar and non-clamping, in lung tissue after 6 h; IR group with the outside retaining direct line of non-traumatic microvascular clamp clamp far side of the heart hilum, hilar block 45 min, and then open lung tissue after 6 h; SF group in front of 30 min occlusion pulmonary venous injection of SF 10 mL/kg. Lung tissue apoptosis rate was detected by flow cytometry, and lung tissue Bcl-2/Bax, Fas/FasL gene expression were detected by in situ hybridization. RESULTS： Compared with the Sh group, IR group and SF group of lung cell apoptosis and Bcl-2/Bax, Fas/FasL gene expression was significantly increased, there were statistically significant difference （P〈0.05）. Compared with the IR group, SF lung cell apoptosis and Bcl-2/Bax, Fas/FasL expression decreased, there were also statistically significant difference （P 〈 0.05）. CONCLUSION： Shenfu injection was Iossed of lung ischemia-reperfusion has a protective effect, with inhibition of lung tissue Bcl-2/Bax, Fas/FasL gene expression, decreased apoptosis.

关 键 词：肺再灌注损伤 参附注射液 细胞凋亡 BCL-2基因 FAS基因 

分 类 号：R655.3[医药卫生—外科学]