肠安康新方胃肠分解给药对大鼠乙酸性结肠炎的影响  

Influence of gastrointestinal decomposition delivery of New Chang'ankang Formula in rats with acetic acid-induced ulcerative colitis

作　　者：于海食[1] 洪缨[1] 王玉蓉[1] 陈浩[1] 董世芬[1] 万雪梅[1] 

机构地区：[1]北京中医药大学中药学院,北京100102

出　　处：《北京中医药大学学报》2010年第6期385-389,393,共6页Journal of Beijing University of Traditional Chinese Medicine

基　　金：国家科技支撑计划资助项目(No.2006BAI09B08)

摘　　要：目的以肠安康新方(黄芪提取物、盐酸小檗碱、木香提取物)为载体,通过观察肠安康新方对大鼠胃肠分解给药——黄芪提取物灌胃给药,盐酸小檗碱、木香提取物灌肠给药,与新方单独灌胃给药对大鼠乙酸性溃疡性结肠炎(UC)模型的影响,研究肠安康新方结肠定位给药在治疗UC中的可行性。方法制备大鼠乙酸性结肠炎模型,实验设正常对照组,模型对照组,SASP组,口服给药高、中、低给药组,定位给药高、中、低给药组。造模后24h开始给药,每天1次,连续给药6d,从造模开始至实验结束共8d。实验过程每日记录大便隐血(OB)变化情况;实验结束后称量全结肠重量,测量全结肠长度、宽度,并计算肠重指数(肠重指数=全结肠重量/全结肠长度),评价结肠黏膜损伤指数(CMDI)和黏膜病理组织学(HS)。结果在改善大鼠结肠长度、结肠重量、结肠宽度上,肠安康新方胃肠分解给药与单独灌胃给药相近;在改善大鼠大便隐血、肠重指数、CMDI、HS上,肠安康新方分解给药优于单独灌胃给药。结论肠安康新方胃肠分解给药对乙酸性结肠炎大鼠结肠黏膜损伤具有保护作用,且优于肠安康新方单独灌胃给药。Objective To study the feasibility of colon-specific delivery of New Chang＇ankang Formula in the treatment of ulcerative colitis （ UC） . The study was conducted through observing the influences,on rat model of acetic acid-induced UC,of gastrointestinal decomposition delivery of New Chang＇ankang Formula,intragastrical delivery of the exact of Huangqi （ Radix Astragalus） ,intraintestinal delivery of berberin hydrochloride and extract of Muxiang （ Radix Aucklandiae） ,and intragastrical delivery of New Chang＇ankang Formula singly. The study took New Chang＇ankang Formula （ exacts of Huangqi and Muxiang,and berberin hydrochloride） as the carrier. Methods The rat model of acetic acid-induced UC was established and then the rats were divided into the normal control group,model control group, Sulfasalazine Tablets （ SASP） group,high-dose,mid-dose and low-dose groups of oral delivery,and high-dose,mid-dose and low-dose groups of colon-specific delivery. The medicinals were given to relevant groups 24 hours after the establishment of the model once a day for 6 days. The changes of fecal occult blood （ OB） were recorded every day. After the test ended,the colonic weight was weighed,the colonic length and width were measured,colonic weight index （ colonic weight/colonic length） was calculated and colonic mucosal damage index （ CMDI） and histological score （ HS） were reviewed. Results The effects of gastrointestinal decomposition delivery of New Chang＇ankang Formula was as that of the same as its intragastrical delivery singly in the improvement of colonic length,weight and width,and was superior in the improvement of OB,colonic weight index,CMDI and HS. Conclusion The gastrointestinal decomposition delivery of New Chang＇ankang Formula has a better protective effect on colonic mucosa in rats with acetic acid-induced UC.

关 键 词：肠安康新方 结肠定位给药 溃疡性结肠炎 乙酸 大鼠 

分 类 号：R285.5[医药卫生—中药学]