补肾活血中药对大鼠慢性高眼压模型视网膜病理改变的影响  

Influences of Chinese herbal remedies with actions of tonifying kidney and activating blood circulation on retinal pathological changes in rat model of chronic elevated intraocular pressure

作　　者：李翔[1] 毛欣[1] 张富文[1] 

机构地区：[1]成都中医药大学附属医院眼科,四川610072

出　　处：《北京中医药大学学报》2010年第6期390-393,I0001,共5页Journal of Beijing University of Traditional Chinese Medicine

基　　金：四川省中医药管理局资助项目(No.2006-C-SZYJ-08)

摘　　要：目的观察补肾活血中药对大鼠慢性高眼压(EIOP)模型视网膜病理改变的影响,并对其作用机理进行初步探讨。方法采用烙闭上巩膜静脉法,制作大鼠慢性EIOP模型,随机分为5组:模型组,补肾活血高剂量组、中剂量组、低剂量组,空白组。连续灌胃8周,并于8周末处死大鼠,观察其对EIOP大鼠眼压(IOP),视网膜神经节细胞(RGCs)和神经纤维层(RNFL)病理形态学变化及RGCs凋亡率的影响。结果①造模后大鼠IOP明显升高,在造模后8周(实验结束)IOP仍为造模前的3倍,补肾活血中药有轻度的降眼压的作用。②模型组大鼠RNFL和神经细胞层(RGCL)厚度为空白组大鼠的1/2,而各治疗组大鼠RNFL和RGCL厚度均高于模型组,提示补肾活血中药可以抑制大鼠慢性EIOP模型RGCs和RNFL的缺损。③模型组大鼠RGCs凋亡率约为空白组的3倍,各治疗组大鼠RGCs凋亡率均低于模型组,提示补肾活血中药对慢性EIOP大鼠RGCs凋亡有明显的抑制作用。结论补肾活血中药可以保护大鼠慢性EIOP模型视功能。Objective To observe the influences of Chinese herbal remedies （ CHR） with actions of tonifying kidney and activating blood circulation on retinal pathological changes in rat model of chronic elevated intraocular pressure （ EIOP） and investigate the mechanism. Methods The rat model of chronic EIOP was established by using cauterization of the episcleral veins,and then the rats were randomly divided into the model group,groups of high-dose,mid-dose and low-dose CHR （ treatment groups） and blank group. The rats were given CHR intragastrically for 8 weeks,and then sacrificed at the end of the 8th week. The intraocular pressure （ IOP） ,pathomorphological changes of retinal ganglion cells （ RGCs） and retinal nerve fiber layer （ RNFL） ,and apoptosis rate of RGC were observed. Results ① IOP increased significantly in rats after modeling and was 3 times higher than that before modeling after modeling for 8 weeks （ test finished） . CHR had mild effect to reduce IOP. ②The thicknesses of RNFL and RGCs layer （ RGCL） in the model group was half of those in the blank group,which was higher in all treatment groups than that in the model group,indicating that CHR could inhibit the lesion of RGCs and RNFL. ③The apoptosis rate of RGCs in the model group was 3 times higher than that in the blank group,which was lower in all treatment groups than that in the model group,indicating that CHR had significant inhibitory effect on RGCs apoptosis. Conclusion CHR with the actions of tonifying kidneyand activating blood circulation can protect the vision of rats with chronic EIOP.

关 键 词：补肾活血 慢性高眼压模型 病理 大鼠 

分 类 号：R285.5[医药卫生—中药学]