扶正祛毒法延缓肝纤维化进展的时机探讨  

Occasion of postponing hepatic fibrosis with therapy of reinforcing the healthy qi and eliminating toxin

作　　者：张立平[1] 郭子宁[1] 赵晶晶[1] 宋莹莹[1] 张志辰[1] 郑宏[1] 

机构地区：[1]北京中医药大学,北京100029

出　　处：《北京中医药大学学报》2010年第6期406-410,I0002,共6页Journal of Beijing University of Traditional Chinese Medicine

基　　金：国家中医药管理局留学回国人员科技活动择优资助项目

摘　　要：目的探讨扶正祛毒法延缓肝纤维化进展的最佳给药时机。方法采用CCl4皮下注射12周的方法制作大鼠肝纤维化模型,中药组给予芪莲纤化汤治疗,并依据给药时间不同分为造模第1周(芪莲1组)、第4周(芪莲2组)、第7周(芪莲3组)3个给药组,西药治疗组(DDB组)从第1周的第1天开始给予联苯双酯滴丸治疗,观察不同组别、不同时期的血清谷丙转氨酶(ALT)、谷草转氨酶(AST)、血清白蛋白(ALB)3项肝脏功能生化指标和肝组织病理。结果注射CCl4后,模型组大鼠一般状况逐渐变差,体重较正常组减轻,后期有腹水出现;第6周开始转氨酶显著升高,第9周末血清白蛋白明显下降;在第6周后出现明显的纤维间隔,肝小叶被破坏、紊乱,9周末肝内假小叶广泛形成。芪莲1组与DDB治疗组、模型组大鼠相比较:芪莲1组肝功能明显好于同期的其他各组,纤维化程度轻。芪莲1组与芪莲2组、芪莲3组大鼠相比较:芪莲1组肝功能好于其他2组。芪莲2组以上观测指标均好于芪莲3组。结论具扶正祛毒功用的芪莲纤化汤对CCl4模型大鼠纤维化进展有明显延缓作用,并且干预得越早越好。Objective To investigate the best occasion of postponing hepatic fibrosis with the therapy of reinforcing the healthy qi and eliminating toxin. Methods The rat model of hepatic fibrosis was established by the hypodermic injection of CCl4. Qilianqianhua Decoction （ QL） was given to the rats, respectively,on the first week （ QL1 group） ,forth week （ QL2 group） and seventh week （ QL3 group） after the modeling. The control group was given dimethyl diphenyl bicarboxylate （ DDB group） on the first day of the first week. The changes of levels of ATL,AST and ALB,and hepatic histopathology were observed in all groups and periods. Results After injecting CCI4,in the rats of the model group,the general condition became poorer and the body weight decreased compared with the rats of the normal group,and ascites was observed in later period. On the 6th week aminopherase increased significantly and on the 9th week serum albumin level decreased significantly. After 6 weeks obvious fibrous septum,and disorder of hepatic lobules were observed,and at the end of 9th week peudolobuli were generated extensively. The results of comparison among the OL1 group,DDB group and model group showed that the hepatic function was much better and fibrosis was to a lesser degree in the QL1 groups than that in the other groups. The results of comparison among the OL1 group,OL2 group and OL3 group showed that the hepatic function was better in the OL1 group than that in the OL2 group and OL3 group,and the aboveobserving indexes were better in the OL2 group than those in the OL3 group. Conclusion Qilianxianhua Decoction,with the actions of reinforcing the healthy qi and eliminating toxin,can postpone the development of hepatic fibrosis in rat model of hepatic fibrosis induced by CCl4. The earlier the therapy is used to intervene hepatic fibrosis,the better the effect is.

关 键 词：肝纤维化 扶正祛毒法 芪莲纤化汤 干预时机 大鼠 

分 类 号：R285.5[医药卫生—中药学]