藤梨根提取物对小鼠染色体DNA抗诱变作用的初步研究  

Anti-mutagenesis effect of extract of Tengligen (Radix Actinidiae Chinensis) on chromosome DNA in mice

作　　者：崔向清[1] 杨向竹[1] 黄春芳[1] 刘欢[1] 郭雁[1] 

机构地区：[1]北京中医药大学基础医学院,北京100029

出　　处：《北京中医药大学学报》2010年第6期411-412,416,共3页Journal of Beijing University of Traditional Chinese Medicine

摘　　要：目的观察藤梨根提取物对小鼠染色体DNA抗诱变效应,为藤梨根在化疗期间减毒作用提供动物实验依据。方法将昆明种小鼠随机分组,用环磷酰胺(CP)诱变DNA,低、高剂量藤梨根提取物灌胃4d,通过观察小鼠骨髓细胞嗜多染红细胞(PCE)微核(MN)的发生情况,研究藤梨根的抗诱变作用。结果低剂量的藤梨根提取物对环磷酰胺诱发的小鼠骨髓细胞微核发生有明显的抑制效果,MN发生率与模型组比较差异显著,P<0.05。结论藤梨根提取物对环磷酰胺诱变导致的DNA损伤具有保护作用。Objective To observe the anti-mutagenesis effect of extract of Tengligen （ Radix Actinidiae Chinensis） on chromosome DNA in mice,and provide the experimental base in animals for the attenuation of Tengligen during chemotherapy. Method All Kunming mice were randomly divided into groups,and the mutagenesis of DNA was induced with cyclophosphane （ CP） . The mice were given low-dose and high-dose Tengligen extract intragastrically for 4 days. The anti-mutagenesis effect of Tengligen was studied by observing the genesis of micronucleus （ MN） of polychromatic erythrocyte （ PCE） . Result The extract of Tengligen in low dose had a significant inhibitory effect on the genesis of MN of PCE. The genesis rate of MN in the low-dose group was significantly different to that in the model group （ P 0. 05） . Conclusion The extract of Tengligen has a preventive effect on DNA lesion induced by CP.

关 键 词：藤梨根 环磷酰胺 抗诱变 微核 小鼠 

分 类 号：R285.5[医药卫生—中药学]