脂泰胶囊对实验性动脉粥样硬化家兔内皮素及一氧化氮合酶基因表达的影响  

Influence of Zhitai Capsule on the Expression of Endothelins and Nitric Oxide Synthase in Experimental Atherosclerosis of Rabbits

作　　者：李亚俊[1,2] 宋剑南[1,2] 周瑕菁[1,2] 牛晓红 王宇辉[1,2] 金红 王少君[1,2] 

机构地区：[1]中国中医研究院基础理论研究所 [2]中日友好医院临床医学研究所

出　　处：《中国动脉硬化杂志》1999年第1期4-8,共5页Chinese Journal of Arteriosclerosis

基　　金：国家自然科学基金

摘　　要：为在基因水平上探讨痰瘀同治方药脂泰胶囊保护血管内皮、抗动脉粥样硬化形成和发展的机理，采用烟酰胺磷酸腺嘌呤二核苷酸—硫锌酰胺脱氢酶组织化学染色和原位杂交法观察了脂泰胶囊对高脂血症家兔主动脉壁一氧化氮合酶活性及其ｍＲＮＡ表达和内皮素ｍＲＮＡ表达的影响，采用阳性积分法处理数据。结果发现，脂泰胶囊组家兔胸主动脉一氧化氮合酶活性（１．３８±０．６３）明显高于高脂组（０．４０±０．２４）（Ｐ＜０．０１）；脂泰胶囊组家兔胸主动脉一氧化氮合酶ｍＲＮＡ表达（１．３８±０．４８）明显高于高脂组（１．００±０．００）（Ｐ＜０．０５）；脂泰胶囊组家兔胸主动脉内皮素ｍＲＮＡ表达（２．４３±０．５３）明显低于高脂组（３．３３±０．８２）（Ｐ＜０．０５）。此外，脂泰胶囊可以阻止斑块增厚，减少斑块内脂质坏死中心的形成。提示脂泰胶囊能明显升高高脂状态下主动脉血管壁的一氧化氮合酶活性及其ｍＲＮＡ表达，降低内皮素ｍＲＮＡ表达。脂泰胶囊抗动脉粥样硬化形成和发展的作用机制之一可能是通过调控一氧化氮合酶ｍＲＮＡ和内皮素ｍＲＮＡ的表达，从而达到维持一氧化氮合酶的正常活性及一氧化氮的正常水平来实现的。Aim To investigate the molecular mechanism of the Zhitai Capsule, which is a phelgm and stasis treating prescription, protecting vascular endothelial cells and against atherosclerosis. Methods 24 male New Zeland White rabbits were divided into four groups: the normal control group, the hypercholesterol group, the Gimfibrozil group and the Zhitai group. Four months later, the rabbits were killed and the aortas were fixed. Using NADPH diaphorase histochemistry and in situ hybridization, we have studied the activity and mRNA expression of nitric oxide synthase(NOS),as well as the mRNA expression of endothelin in aorta. Results The NOS activity in the aortas of Zhitai group (1.38±0.63) were significantly higher than that of hypercholesterol group(0.40±0.24)(P<0.01); that the NOS mRNA expression in the aortas of Zhitai group(1.38±0.48) were much higher than that of hypercholesterol group(1.00±0.00)(P<0.05); and that the NOS mRNA expression in the aortas of Zhitai group (2.43±0.53) were much lower than that of hypercholesterol group(3.33±0.82)(P<0.05). The Zhitai capsule can prevent the activity and mRNA expression of NOS in advanced atherosclerosis from decending, the mRNA expression of endothelin in advanced atherosclerosis from increasing, and decrease the lipid necrosis core. Conclusion Zhitai capsual's antiatherosclerosis role was correlated with it′s effect on mRNA expression of nitric oxide synthase and endothelin.

关 键 词：动脉粥样硬化 内皮素 一氧化氮合酶 脂泰胶囊 

分 类 号：R259.435[医药卫生—中西医结合] R285.5[医药卫生—中医内科学]