白藜三醇对人低密度脂蛋白氧化修饰的影响  

Effect of Resveratrol on Oxidative Modification of Human Low Density Lipoprotein

作　　者：邹建刚[1] 黄元铸[1] 陈琪[1] 魏恩会[1] 曹克将[1] 

机构地区：[1]南京医科大学

出　　处：《中国动脉硬化杂志》1999年第1期13-16,共4页Chinese Journal of Arteriosclerosis

摘　　要：为探讨白藜三醇的抗脂质氧化作用及其可能机制，采用Ｃｕ２＋和２，２＇－盐酸脒基丙烷两种不同的氧化体系诱导正常人低密度脂蛋白的氧化反应，应用硫代巴比妥酸法测定低密度脂蛋白氧化过程中硫代巴比妥酸反应物质和氧化型低密度脂蛋白的相对电泳迁移率的变化。结果发现５０μｍｏｌ／Ｌ白藜三醇可使Ｃｕ２＋介导的低密度脂蛋白氧化反应明显减弱，其硫代巴比妥酸反应物质和相对电泳迁移率分别下降７０．５％和４２．３％（Ｐ＜０．０１）；同时白藜三醇使Ｃｕ２＋和２，２＇－盐酸脒基丙烷诱导的低密度脂蛋白氧化反应潜伏期延长。上述结果提示白藜三醇具有抑制低密度脂蛋白氧化反应作用，推测其作用机制可能与捕获自由基等有关。Aim The antioxidation effect of resveratrol, a polyphenolic compound in red wine, on the oxidation of human low density lipoprotein (LDL) were systematically investigated by two different oxidation systems. Methods Oxidation of LDL was induced by adding Cu 2+ or azo compound. The extent of LDL modification was assessed by measuring the formation of thiobarbituric acid reactive substances (TBARS) and the relative electropherosis mobilities (REM) to native LDL. Results Treatment of LDL with resveratrol (50 μmol/L) reduced the production of TBARS and REM of LDL during Cu 2+ induced oxidation by 70.5% and 42.3%, respectively (P<0.01). The lag phase of LDL oxidation induced by copper ion or azo compound was delayed. Conclusion Resveratrol could protect LDL against both Cu 2+ induced and azo compound initiated oxidative modification in vitro, which might be contributed by its free radicals scavenging capacity.

关 键 词：白藜三醇 低密度脂蛋白 抗氧化剂 动脉粥样硬化 

分 类 号：R543.501[医药卫生—心血管疾病] TS262[医药卫生—内科学]