雄黄及微生物炮制液砷形态和毒性分析  

Speciation Analysis and Toxicity of Arsenic in Realgar Bioleaching Solution

作　　者：张景红[1,2] 樊秦[3] 李红玉[1] 

机构地区：[1]兰州大学生命学院,甘肃兰州730000 [2]华侨大学教育部分子药物研究工程中心,福建泉州362021 [3]甘肃中医学院动物实验中心,甘肃兰州730030

出　　处：《中药材》2010年第5期684-688,共5页Journal of Chinese Medicinal Materials

基　　金：甘肃省科技攻关项目(2GS064-A43-019-02);福建省自然科学基金(2008J0102);泉州市科学基金(2008Z76);华侨大学人才引进启动项目(BS105)

摘　　要：目的:对雄黄及微生物炮制液砷形态和毒性进行分析,旨在阐明雄黄炮制液中毒性和砷形态的关系。方法:采用毛细管电泳法对三种雄黄微生物炮制液中砷的形态进行分离分析;以常规炮制雄黄作对照,分别测定三种雄黄微生物炮制液和亚砷酸的急性毒性。结果:雄黄微生物炮制液含有三种形态的砷(iAsⅢ、iAsV和MMAV),而且其所含砷的形态、含量随炮制方法变化差异显著,MMAV随着含量的增高,其毒性明显降低。结论:雄黄炮制后砷的形态和毒性密切相关。Objective：To analyze speciation and toxicity of arsenic in realgar bioleaching solution,and to explore its possible relation between speciation and toxicity.Methods：Capillary zone electrophoresis（CZE）can be used quickly and simply onto the simultaneous separation and quantitative determination of the speciation of arsenic in realgar bioleaching solution.The effects of three different realgar bioleaching liquids on the acute toxicity level of rat were also investigated as well.Parallel experiments with traditional processing realgar via gastric irrigation were conducted for comparison.Results：There are three different arsenic species in realgar bioleaching solution（iAsⅢ、iAsV和MMAV）,and the processing method largely affected speciation and toxicity of arsenic of it.It was found that the toxicity level was decreased through increasing the amount of MMAV.Conclusion：These results indicate that the species of arsenic may be tightly relationship to its toxicity in realgar bioleaching solution.

关 键 词：雄黄 微生物炮制 砷 形态分析 毒性 

分 类 号：R283.1[医药卫生—中药学]