番石榴叶止泻作用有效部位的筛选  

Screening of Anti-Diarrhea Effective Fractions from Guava Leaf

作　　者：卢文彪[1] 张倍恩[1] 王静[1] 卢瑞芯[1] 李茹柳[1] 陈蔚文[1] 

机构地区：[1]广州中医药大学,广东广州510405

出　　处：《中药材》2010年第5期732-735,共4页Journal of Chinese Medicinal Materials

基　　金：广州中医药大学邓铁涛基金课题(004010)

摘　　要：目的:筛选番石榴叶止泻作用有效部位并建立质量控制方法。方法:以番泻叶致小鼠腹泻模型评价药效,采用溶剂提取分离方法逐级制备药材提取物系列试样并筛选药效较强的部位;采用HPLC测定试样中与药效相关的成分含量。结果:番石榴叶中等极性的苷类部位呈较强的止泻作用;苷类部位的药效与其结合态槲皮素含量呈相关性。结论:中等极性的苷类部位是番石榴叶止泻作用的有效部位;槲皮素苷类含量测定可用于番石榴叶及其提取物的质量控制。Objective：To screen the fractions from guava leaf to ascertain the effective anti-diarrhea fraction and to establish the method of quality control.Methods：A series of samples from guava leaf were prepared through extraction and separation by solvents progressively,and their effect on the mice diarrhea models induced by senna decoction were tested individually;and the constituent correlated with the effect of the samples on the models was determined by HPLC.Results：The effect of the fractions with moderate-polar glucosides from guava leaf was better than that of the other fractions;the effect of the fractions with the glucosides was correlated with the content of the quercetin glucosides in the fractions.Conclusion：The fractions with the moderate-polar glucosides is the effective anti-diarrhea fraction of guava leaf;determination of the quercetin glucosides can be used for quality control of guava leaf and its extracts.

关 键 词：番石榴叶 腹泻 有效部位 槲皮素苷类 高效液相色谱 

分 类 号：R285.5[医药卫生—中药学] R284.2[医药卫生—中医学]