维生素B2片快速检验的近红外漫反射光谱法定量模型初探  

A rapid and nondestructive method for determinating vitamin B2 tablets by near-infrared diffuse reflectance spectroscopy with fiber-optical probe

作　　者：朱健永 李小峰[1] 郑峰[1] 

机构地区：[1]安徽省淮北市食品药品检验所,安徽淮北235000

出　　处：《安徽医药》2010年第8期902-904,共3页Anhui Medical and Pharmaceutical Journal

摘　　要：目的初步建立近红外漫反射光纤光谱法测定维生素B2片含量的定量模型。方法利用＂药品快检车＂配备的近红外光谱仪,用光纤探头采集近红外漫反射光纤光谱,对国内不同生产企业的维生素B2片样品建立了定量分析模型。应用最小二乘法（PLS）,在7 116.4~4 468.5 cm-1范围内,光谱采用一阶导数与多元散射校正（MSC）预处理,建立回归校正模型。结果维生素B2在57.5~87.5 mg.g-1的浓度范围内,定量分析模型的相关系数R2为0.9378,内部交叉验证均方差（RMSECV）为1.79,预测均方差（RMSEP）为1.36。结论近红外漫反射光纤光谱法能够实现维生素B2片的无损定量分析,可用于快速筛查。Aim To apply near infrared diffuse reflectance spectrophotometry （NIRDRS） and chemometrics for the quantitative analysis of Vitamin B2 tablets. Method Near-infrared spectra （NIR） in the range of 12 000 -4 000 cm^-1 were recorded by a fibre optic probe for the Vitamin B2 tablets containing riboflavin in the content of 57.5 - 87.5 mg · g^-1. Different spectra pretreatments methods were compared. Finally,NIR spectra in the wavelength range of 7 116.4 -4 468.5 cm^-1 were selected to set up the calibration curve by the Partial Least squares Regression（PLSR） method, pre-treating with the first derivative combining multiplicative signal correction （MSC） method. Results The relation coefficients R2 was 0.9378 as indicated from the cross-validation, and the root mean square error of cross- validation （RMSECV） was 1.79, the root mean square error of prediction （RMSEP） 1.36. Conclusion The proposed method is robust, and could be used in the fast analysis of Vitamin B2 tablets.

关 键 词：近红外 漫反射光谱法 快速检验 维生素B2 含量测定 

分 类 号：R927[医药卫生—药学]