Molecular Analysis and Expression Patterns of Four 14-3-3 Genes from Brassica napus L.  

作　　者：ZHAN Gao-miao,TONG Jin,WANG Han-zhong and HUA Wei Key Laboratory for Biological Sciences of Oil Crops,Ministry of Agriculture/Oil Crops Research Institute,Chinese Academy of Agricultural Sciences,Wuhan 430062,P.R.China 

出　　处：《Agricultural Sciences in China》2010年第7期942-950,共9页中国农业科学（英文版）

基　　金：supported by the National High Technology Research and Development Program of China(2007AA10Z124 and 2006AA10A113)

摘　　要：In eukaryotes,14-3-3 proteins constitute a family of ubiquitous regulatory molecules.They play very important roles in many cell processes.However,their enconding genes and roles in plants remain to be elucidated.In this paper,four 14-3-3 genes from Brassica napus L.were obtained by randomly sequencing a full-length cDNA library and named as Bn1433-1-4 respectively.The phylogenetic comparison with Arabidopsis 14-3-3 family showed that Bn1433-1 and Bn1433-2 belonged to Episilon group while Bn1433-3 and Bn1433-4 belonged to non-Episilon group.The transcript levels of four Bn1433 genes were analyzed in different organs,various stress conditions and some hormone treatments by real-time PCR.The result showed that all the Bn1433s were expressed constitutively in roots,stems,leaves and immature seeds except that Bn1433-4 exhibited strong expression in immature seeds of 14 and 28 DAF（day after flowering）.By the analysis of real-time PCR,different expression patterns of Bn1433s were studied in various stress conditions and hormone treatments.It was suggested that the functions of Bn1433s are diverse and they are involved in the regulation of various stresses and hormones treatments.In eukaryotes,14-3-3 proteins constitute a family of ubiquitous regulatory molecules.They play very important roles in many cell processes.However,their enconding genes and roles in plants remain to be elucidated.In this paper,four 14-3-3 genes from Brassica napus L.were obtained by randomly sequencing a full-length cDNA library and named as Bn1433-1-4 respectively.The phylogenetic comparison with Arabidopsis 14-3-3 family showed that Bn1433-1 and Bn1433-2 belonged to Episilon group while Bn1433-3 and Bn1433-4 belonged to non-Episilon group.The transcript levels of four Bn1433 genes were analyzed in different organs,various stress conditions and some hormone treatments by real-time PCR.The result showed that all the Bn1433s were expressed constitutively in roots,stems,leaves and immature seeds except that Bn1433-4 exhibited strong expression in immature seeds of 14 and 28 DAF（day after flowering）.By the analysis of real-time PCR,different expression patterns of Bn1433s were studied in various stress conditions and hormone treatments.It was suggested that the functions of Bn1433s are diverse and they are involved in the regulation of various stresses and hormones treatments.

关 键 词：14-3-3 expression pattem abiotic stress Brassica napus L. 

分 类 号：S565.4[农业科学—作物学] S858.23