脉君安片对自发性高血压大鼠内皮素-1和一氧化氮合酶表达的影响  

Effects of Maijun'an Tablets on expression of ET-1 and NOS in spontaneous hypertensive rats

作　　者：张继业[1] 王宇[1] 梁继超[1] 韩凤梅[1] 陈勇[1] 

机构地区：[1]湖北大学中药生物技术省重点实验室,武汉430062

出　　处：《中华中医药杂志》2010年第7期1073-1076,共4页China Journal of Traditional Chinese Medicine and Pharmacy

基　　金：2008年湖北省高等学校优秀中青年团队计划项目(No.T200807)~~

摘　　要：目的:研究脉君安片对自发性高血压大鼠相关组织中内皮素-1(ET-1)和一氧化氮合酶(NOS)表达的影响,探讨其降压机制。方法:12只成年自发性高血压大鼠随机分为给药组和对照组,给药组灌胃模拟脉君安片(氢氯噻嗪10mg·kg-1.d-1+葛根素36mg·kg-1.d-1+钩藤总碱122mg·kg-1.d-1),对照组灌胃等量去离子水。每周测血压1次,4周后处死动物,分别检测组织与血浆中ET-1和NOS mRNA和蛋白质的表达量。结果:与对照组相比,用药4周后给药组降压效果显著(P<0.05),肝、肾与血浆中NOS基因与蛋白表达水平上调,而ET-1的基因与蛋白表达水平下调。结论:脉君安片可通过上调NOS的表达和下调ET-1的表达,降低自发性高血压大鼠的血压。Objective：To investigate the effects of Maijun＇an Tablets on expression of endothelin-1（ET-1） and nitricoxide synthase（NOS） in tissues and plasma of spontaneous hypertensive rats （SHR）. Methods：12 spontaneous hypertensive rats （SHR） were divided randomly into normal control group and drug administration group. Rats in drug administration group were orally given with Maijun＇an Tablets （hydrochlorothiazide 10 mg·kg^-1.d^-1,puerarin 36 mg·kg^-1.d^-1 and total alkaloid of rhynchophylla 122 mg·kg^-1.d^-1） once a day for 4 weeks,and rats in control group were orally dosed with equal amount of physiological saline. All animals were sacrificed after 4 weeks. The mRNA and protein contents of ET-1 and NOS in plasma,liver,kidnay and blood vessel were determined. Results：Compared with the control group,Maijun＇an Tablets can effectively reduce blood pressure of SHRs,regulate down the content of ET-1 and regulate up the content of NOS at mRNA and protein levels. Conclusion：The up-regulation of NOS and down-regulation of ET-1 at mRNA and protein levels were the major antihypertensive machnisam of Maijun＇an Tablets for SHRs.

关 键 词：脉君安片 自发性高血压大鼠 一氧化氮合酶 内皮素-1 

分 类 号：R285.5[医药卫生—中药学]