气虚血瘀证与Balb/c小鼠结肠癌血行转移相关性实验研究  

Experimental Study of the Qi Deficiency and Blood Stasis Syndrome Related to Artificial Hematogenous Metastasis of Conlon Cancer in Balb/c Rats

作　　者：姚庆华[1] 楼亭[2] 郭勇[1] 

机构地区：[1]浙江省中医院,杭州310006 [2]浙江中医药大学第一临床医学院,杭州310053

出　　处：《中华中医药杂志》2010年第7期1115-1119,共5页China Journal of Traditional Chinese Medicine and Pharmacy

基　　金：浙江省中医药管理局课题(No.2008CA009)~~

摘　　要：目的:通过建立气虚血瘀证Balb/c小鼠结肠癌人工血行转移的复合模型,研究气虚血瘀证与Balb/c小鼠结肠癌血行转移的相关性。方法:将36只8周龄雌性Balb/c小鼠随机分6组,即空白对照组、血瘀组、肿瘤A组、肿瘤B组、瘀加瘤A组和瘀加瘤B组。肿瘤B组和瘀加瘤B组,进行生存期观察。其余各组第28d处死,检测血黏度及转移灶计数。结果:气虚血瘀造模后小鼠体质量增加缓慢,与空白对照组比较差异有显著性(P<0.01)。血瘀组及瘀加瘤组全血黏度均明显升高,与空白对照组比较差异显著(P<0.01)。瘀加瘤组右肺转移灶计数明显高于肿瘤组,生存期短于肿瘤组,差异有统计学意义(P<0.05)。结论:气虚血瘀证与Balb/c小鼠结肠癌血行转移呈正相关,能促进结肠癌转移,加重肿瘤负荷,缩短生存期。Objective ：To Study the Relationship between the Qi Deficiency and Blood Stasis Syndrome and artificial hematogenous metastasis of colon cancer by establishing a compound model of Qi Deficiency and Blood Stasis Syndrome and artificial hematogenous metastasis of colon cancer. Methods：Thirty six eight-weeks old female Balb/c rats were divided into six groups randomly：control group ,stasis group,tumor groupA、B ,stasis and tumor groupA、B. We observed the survival time of tumor groupB and stasis and tumor groupB. At the 28th day,after killing the mice of the other groups,we detected the whole blood viscosity and the amounts of metastasis tubercle on lung. Results：The weight of the stasis group increase more slowly than control group（P0.01）.The whole blood viscosity of stasis group,stasis and tumor group was more significantly improved than control group（P0.01）.The amounts of metastasis tubercle on lung in stasis and tumor group were more significantly improved than tumor group（P0.05）. The survival time of stasis and tumor group was significantly shoter than tumor group（P0.05）. Conclusion：Qi Deficiency and Dlood Stasis Syndrome can promote the hematogenous metastasis of colon cancer,increase tumor burden,shorten its lifetime.

关 键 词：气虚血瘀证 结肠癌 血行转移 

分 类 号：R273[医药卫生—中西医结合]