人趋化素样因子对大动脉内皮细胞的促增殖和促迁移作用初探  

Effects of human chemokine like factor-1 (CKLF-1) on proliferation and migration of human aortic endothelial cells (HAEC)

作　　者：陈琛[1] 洪涛[1] 盛琴慧[1] 龚艳君[1] 霍勇[1] 

机构地区：[1]北京大学第一医院心内科,100034

出　　处：《中国介入心脏病学杂志》2010年第3期157-160,共4页Chinese Journal of Interventional Cardiology

摘　　要：目的探讨人趋化素样因子对大动脉内皮细胞增殖和迁移的影响。方法构建人趋化素样因子质粒,转染293T细胞,以转染后上清刺激人大动脉内皮细胞HAEC,通过CCK-8法检测不同时间细胞活性,应用transwell法检测48h后迁移细胞数。结果发现转染CKLF1组上清6h增殖率为1.842922,24h增殖率为1.131596,48h增殖率为1.173068,与转染空质粒组(6h增殖率1.562557,24h增殖率0.966689,48h增殖率1.000442)相比可以促进HAEC细胞的增殖,48h迁移细胞CKLF1组(58.0±24.7)个细胞,与PCDNA组(10.6±3.4)个,阴性对照组(14.5±11.6)个相比结果具差异有统计学意义(F=4.535,P<0.05)。结论人趋化素样因子可促进大动脉内皮细胞的增殖和迁移,对新生血管的形成可能有一定作用。Objective To study the effects of human chemokine like factor-1 （CKLF-1） on proliferation and migration of human aortic epithelial cells （HAEC）.Methods CKLF-1 plasmid was transfected to 293T cells.The supernatants of transfected 293T cells were added to HAECs plated in 96-well plates.Cell count kit-8 was used to detect cell calculation.HAECs were plated in upper chambers,and supernatants of transfected 293T cells were added in the lower chambers,then cell migration was detected by calculate the cell numbers in the lower chambers.Results The proliferation rate of CKLF1 group was 1.842922 in 6 hours,1.173068 in 24 hours,and 1.131596 in 48 hours,which showed CKLF1 could promote cell proliferation compared to the empty vector transfected group （6 hours 1.562557,24 hour 0.966689,48 hours 1.000442）.The 48 h-migrated cells in CKLF1 group was 58.0±24.7,which was significantly different compared to the other two groups （PCDNA group 10.6 ± 3.4,negative control group,14.5±11.6 unit,P0.05）.Conclusion CKLF1 can promote the proliferation and migration of HAECs.

关 键 词：人趋化素样因子 大动脉内皮细胞 增殖 迁移 

分 类 号：R392.12[医药卫生—免疫学]