不同培养基对鸡血管内皮祖细胞增殖能力的影响  

The Influences of Media on in vitro Culture of Chicken Vascular Endothelial Progenitor Cells

作　　者：白春雨[1,2] 侯玲玲[3] 宫雪莲[2] 马月辉[2] 关伟军[2] 庞全海[1] 

机构地区：[1]山西农业大学动物科技学院,太谷030801 [2]中国农业科学院北京畜牧兽医研究所,北京100193 [3]北京交通大学生命科学与生物工程研究院,北京100044

出　　处：《中国细胞生物学学报》2010年第3期387-391,共5页Chinese Journal of Cell Biology

基　　金：国家科技支撑项目(No.2006BAD 1 3B08;No.2008BADB2B01);"863"课题基金(No.2006AA10Z198;No.2007AA10Z170);转基因生物新品种培育科技重大专项(No.2008ZX08009-003)资助项目~~

摘　　要：采用密度梯度离心法分离鸡血管内皮祖细胞,通过Dil-acLDL和FITC-UAEI双阳性鉴定血管内皮祖细胞。在三种不同的培养基即培养基A、培养基B、培养基C中进行体外扩增培养,经生长曲线、扩增能力和克隆形成数量三方面评价这三种培养基,结果表明培养基A在体外最适合鸡血管内皮祖细胞的体外扩增。细胞生长曲线呈现明显的"S"形,细胞群体倍增时间约为23.4 h,体外扩增可至9代,细胞克隆形成总数为38。该研究为血管内皮祖细胞体外增值与临床应用奠定了实验依据。Chicken vascular endothelial progenitor cells （VEPCs） were isolated with density gradient centrifugation and identified with Dil-acLDL and FITC-UEA1 fluorescences. Media A, B and C were compared in terms of growth kinetics, expansion capacity and clonogenic numbers of the cultured VEPCs. The results suggested that medium A is optimal for the in vitro culture, in which the cells living exhibited an apparent ＂S＂ shaped growth curve and a population doubling time of 23.4 h, could be subcultured to passage 9 at most and formed 38 colonies. This study laid an experimental foundation for in vitro expansion and clinical application of vascular endothelial progenitor cells.

关 键 词：鸡 血管内皮祖细胞 干细胞 分离培养 体外扩增 鉴定 

分 类 号：R329[医药卫生—人体解剖和组织胚胎学]