小鼠EL-4淋巴瘤细胞中靶向TERT的RNA干涉  

mTERT-targeted RNA Interference in EL-4 Lymphoma Cells of Mice

作　　者：张敏[1] 郭欣欣[1] 奚耕思[1] 

机构地区：[1]陕西师范大学生命科学学院,西安710062

出　　处：《中国细胞生物学学报》2010年第3期399-403,共5页Chinese Journal of Cell Biology

基　　金：陕西省自然科学基金(No.2004C_107);陕西师范大学重点科研项目资助~~

摘　　要：为了初步研究小鼠肿瘤细胞端粒酶催化亚基mTERT的表达抑制及其对细胞增殖的影响,本研究应用RNA干涉方法,将体外转录合成的靶向mTERT的短发夹RNA(shRNA)转染至表mTERT的小鼠EL-4淋巴瘤细胞。RT-PCR和Western blot检测结果显示,靶向mTERT的shRNA转染到小鼠EL-4细胞后,有效抑制了细胞中内源性mTERTmRNA和蛋白的表达。MTT实验显示,转染后细胞数量未见明显差异,可能缘于TERT抑制导致的表型滞后现象。In order to investigate the expression inhibition and gene regulation of telomerase reverse transcriptase（TERT） in tumour cells of mice, designed short hairpin RNA targeted mTERT was synthesized and transfected into EL-4 lymphoma cells of mice through cationic liposomes. Further analysis of RT-PCR and Western blot indicated that the mTERT specialized shRNA effectively repressed the expressions of endogenous mTERT mRNA and protein after transfection, which made the targeted genes partially silent. MTT experiments demonstrated that the amount of EL-4 cells did not show significant difference between the sample treated with TERT specialized shRNA and the control, which may due to the phenotypic lag phenomenon caused by the inhibition of telomerase.

关 键 词：端粒酶催化亚基 RNA干涉 短发夹RNA 

分 类 号：Q343[生物学—遗传学]