幼年大鼠神经根移植修复对GDNF及其受体影响的研究  

Study of nerve grafting to the change of GDNF and GFRal on neurons after brachial plexus nerve injury in young rats

作　　者：傅阳[1] 陈亮[2] 顾玉东[2] 

机构地区：[1]复旦大学附属中山医院青浦分院骨科,上海201700 [2]复旦大学附属华山医院手外科

出　　处：《中华显微外科杂志》2010年第3期210-212,I0006,共4页Chinese Journal of Microsurgery

基　　金：国家重点基础研究发展计划（973项目,2003CB515305）

摘　　要：目的研究神经移植术修复幼年大鼠臂丛神经损伤后对神经元胶质细胞源性神经营养因子（GDNF）及其受体GFRα1的影响。方法将出生18 d SD大鼠12只等分为2组：颈5切断组，将右侧颈，神经根切除0．3cm;颈5修复组，颈5神经根切除后取腓肠神经移植修复。于术后4周取颈5脊髓和背根神经节．行免疫组织化学染色检测脊髓前角和背根神经节及免疫阳性颗粒的数量，并比较两组间的差异。结果颈5修复组运动和感觉神经元GDNF免疫阳性颗粒数目分别为（786．3±176．84）和（2997．0±357．99）个，颈5切断组运动和感觉神经元阳性颗粒数目分别为（335．0±49．50）和（1632．0±305．55）个，两组差异有统计学意义（P〈0．01）。颈5修复组运动和感觉神经元GFRα1免疫阳性颗粒数目分别（787．5±178．55）和（3111．0±445．72）个，颈5切断组运动和感觉神经元阳性颗粒数目分别为（397．3±41．78）和（1588．3±229．00）个，两组差异有统计学意义（P〈0．01）。结论神经移植术修复幼年大鼠臂丛神经损伤后对近端神经元的保护作用与GDNF及其受体GFRα1增多有关。Objective To investigate the difference of the glial cell line-derived neurotrophic factor and its receptor content of proximal neurons after nerve grafting was used to reconstruct C5 root in young rats. Methods Model of C5 resection was set up in 12 18-day-old SD rats. Experimental animals were divided in to two groups, one group for C5 resection, another for nerve grafting to reconstruct the C5 defection. At 4 weeks postoperatively, the immunohistochemical staining was performed and the number of GDNF and GFRat immunohistochemical positive neurons were calculated respectively. Results The number of GDNF positive neurons in spinal cord and dorsal root ganglion of C5 repairing group was 786.3 ± 176.84 and 2997.0 ±357.99, and that of C5 resection group was 335.0 ±49.50 and 1632.0 ±305.55. On the other hand, the number of GFRal positive neurons in spinal cord and dorsal root ganglion of C5 repairing group was 787.5 ±178.55 and 3111.0 ±445.72, that of the other group was 397.3± 41.78 and 1588.3 ±229.00. The statistical analysis result showed GDNF and GFR immunohistochemical positive neurons in spinal cord and dorsal root ganglion of C5 repairing group was statistically more than that of C5 resection group（P 〈 0.01 ）. Conclusion The neuronal protective effect of nerve grafting after reconstructing brachial plexus nerve injury in young rats may be attributed to the increase of GDNF and its receptor GFRal content of proximal neuron.

关 键 词：臂丛 神经移植 神经元 胶质细胞源性神经营养因子 分娩性臂丛神经麻痹 

分 类 号：R651.3[医药卫生—外科学]