家蝇抗菌肽基因在家蝇幼虫中的空间转录模式  

Spatial transcription pattern of antimicrobial peptide gene in housefly larvae

作　　者：马艳[1] 朱家勇[1] 金小宝[1] 卢雪梅[1] 李小波[1] 褚夫江[1] 

机构地区：[1]广东药学院基础学院广东省生物活性药物重点实验室,广州510006

出　　处：《免疫学杂志》2010年第7期565-568,共4页Immunological Journal

基　　金：国家自然科学基金资助项目(30671832)

摘　　要：目的研究家蝇抗菌肽基因attacin、cecropin、defensin在家蝇幼虫体内的空间转录情况。方法采用体外转录方法合成地高辛标记的cRNA探针,通过原位杂交方法检测微生物诱导前后家蝇幼虫体内attacin、cecropin、defensin变化情况。结果在未诱导的家蝇幼虫体内,体壁、中肠组织均没有检测到attacin、cecropin和defensin转录,在脂肪体中仅检测到cecropin和defensin的弱转录信号;在诱导后的家蝇幼虫中,体壁上检测到attacin和defensin强转录信号,cecropin没有转录,脂肪体中检测到attacin、cecropin和defensin强转录信号,而在中肠组织中检测到cecropin和defensin转录,没有attacin转录。结论经微生物诱导后,家蝇幼虫体内抗菌肽基因转录水平大大提高,为进一步研究家蝇抗菌肽在机体免疫中的作用及其机制打下了基础。The study aimed to study the spatial transcription of the housefly antimicrobial peptides,including attacin,cecropin,and defensin,in the housefly larvae.Digoxin-labeled cRNA probe was synthesized by in vitro transcription,and then used to detect attacin,cecropin,and defensin transcription in unchallenged and challenged housefly larva tissues by in situ hybridization.In the unchallenged larvae,attacin,cecropin,and defensin transcriptions were not found in the epithelia of the body wall and the midgut,while weak transcription of cecropin and defensin was detected in the fat body;in the challenged larvae,stronger transcription signals of attacin and defensin was detected in the epithelia of the body wall,but cecropin was not.Attacin,cecropin,and defensin transcriptions were found in the fat body;cecropin and defensin transcription was detected in the midgut,while attacin was not.The results mean that in the challenged housefly larvae,the transcription of antimicrobial peptides are increased greatly,which is a foundation to further study their role in the immune as well as the mechanism.

关 键 词：家蝇抗菌肽 原位杂交 机体免疫 

分 类 号：Q78[生物学—分子生物学]