不可分型流感嗜血杆菌重组Hap蛋白免疫抗小鼠急性感染的实验研究  

Study on the anti-NTHi infection of Hap recombinant protein in vivo

作　　者：李婉宜[1] 王保宁[1] 左凤琼[1] 曾蔚[1] 丰锋[1] 邝玉[1] 蒋中华[1] 李明远[1] 

机构地区：[1]四川大学华西基础医学与法医学院微生物学教研室

出　　处：《细胞与分子免疫学杂志》2010年第7期635-637,641,共4页Chinese Journal of Cellular and Molecular Immunology

基　　金：四川省卫生厅科研基金资助项目(070137)

摘　　要：目的：观察重组Hap蛋白免疫抗不可分型流感嗜血杆菌（NHTi）感染的免疫保护效果，初步探讨其保护机制。方法：用纯化的Hap重组蛋白与黏膜免疫佐剂霍乱肠毒素B亚单位（CT-B）联合鼻腔免疫C57BL／6小鼠。NHTi琼脂珠悬液对免疫小鼠进行气管内注射，建立小鼠NHTi急性肺部感染模型。NHTi攻击5d后，收集实验小鼠的支气管肺泡灌洗液（BALF）进行白细胞计数，并取其肺组织进行肺部荷菌数检测及肺组织的病理检测。结果：Hap重组蛋白与CT—B的联合免疫能显著降低小鼠BALF中的白细胞总数、中性粒细胞渗出（P〈0．05）及感染小鼠肺组织内NTHi的荷菌数，并减轻感染小鼠支气管、细支气管周围及肺泡内的炎性浸润程度，明显改善其肺组织的病变。结论：鼻腔免疫的Hap重组蛋白在动物体内能发挥良好抗NTHi感染的免疫保护作用，为NTHi新型疫苗的研发提供了理论基础和实验依据。AIM： To observe the immune effect of Hap recombinant protein on murine model of bronchopneumonia infected with NTHi, and explore the mechanism about the anti-NTHi infection. METHODS： The CS？BL/6 mice intranasally immunized with purified Hap recombinant protein and CT-B were challenged by NTHi encased in agar beads. The immunifaction of anti-infection was observed through encocyte counting of BALF, bacteria detection of lung and the pathologyical change of lung tissue. RESULTS： In the challenge with NTHi experiment, the inflammatory exudation of the infected murine and pathological change of lung tissue was relieved by combined immunization of Hap recombinant protein and CT-B, and quantity of NTHi in lung of the infected murine was reduced obviously. CONCLUSION： The Hap recombinant protein also had good ability of anti-NTHi infection in the murine model of NTHi bronchopneumonia. This study could offer the oretical and experimental basis for development of new vaccine against NTHi.

关 键 词：重组Hap蛋白 CT—B NTHi急性肺部感染模型 黏膜免疫 

分 类 号：R378.41[医药卫生—病原生物学]