肥大细胞脱颗粒促进外源树突状细胞向引流淋巴结归巢  

Mast cell degranulation enhances exogenous dendritic cells homing to draining lymph nodes

作　　者：任书荣[1] 徐立斌[2] 吴志远[2] 曲春枫[2] 高美华[2] 杜君[2] 

机构地区：[1]青岛大学医学院免疫学教研室,山东青岛266071 [2]中国医学科学院肿瘤医院／研究所分子肿瘤学国家重点实验室,北京100021

出　　处：《细胞与分子免疫学杂志》2010年第7期638-641,共4页Chinese Journal of Cellular and Molecular Immunology

基　　金：国家自然科学基金面上项目(30872301);中国医学科学院基本科研费青年基金(JK2007B06)

摘　　要：目的：探讨通过诱导局部肥大细胞脱颗粒促进外源骨髓源性树突状细胞（BM-DC）向淋巴结迁移归巢的方法。方法：采用肥大细胞脱颗粒刺激剂compound48／80（e48／80）进行C57BL／6小鼠局部皮肤注射，诱导局部组织肥大细胞脱颗粒。将体外培养的荧光微球标记的同系小鼠BM-DC注射人c48／80或生理盐水处理的局部皮肤，48h收集注射部位引流淋巴结细胞进行CDllc荧光抗体染色，流式细胞术测定外源回输的BM—DC的迁移效果。结果：c48／80注射小鼠皮肤后可有效地促进局部组织肥大细胞脱颗粒，c48／80处理侧局部引流淋巴结中外源BM—DC迁移细胞总数和淋巴结细胞总数较对照侧均明显增多，增多比例分别为67％-4-43％和55％±43％（P〈0．05）。结论：通过诱导局部皮肤肥大细胞脱颗粒，可促进外源BM—DC向淋巴结归巢。AIM： To establish a method of enhancing exogenous bone marrow derived dendritic cells （BM-DC） homing to draining lymph nodes by induction of local mast cell degranulation. METHODS： Compound 48/80 （ c48/80 was injected into C57BL/6 scapular skin to induce local mast cell degranulatJon. BM-DC generated from bone marrow of syngenic mice were labeled with YG-Microspheres and injected into c48/80 or normal saline treated scapular skin. Cells derived from draining lymph nodes （DLN） were pooled together 48 h after BM-DC injection and stained with conjugated anti-CD11c. The efficiency of BM-DC homing to lymph nodes was analyzed by FCM. RESULTS： Mast cell degranulation was locally boosted by c48/80 injection and caused enhancement of the homing of exogenous BM-DC to DLN, with an increase of 67% ± 43%. The total cells in lymph nodes also increased significantly. The fold of increase was 55% ± 43%. CONCLUSION： Exogenous BMDC homing to DLN can be boosted by inducing local skin mast cell degranulation.

关 键 词：树突状细胞 淋巴结归巢 肥大细胞脱颗粒 

分 类 号：R392[医药卫生—免疫学]